Title |
Proteome analysis reveals global response to deletion of mrflbA in Monascus ruber |
Author |
Qingqing Yan1, Zhouwei Zhang2, Yishan Yang1, Fusheng Chen1, and Yanchun Shao1* |
Address |
1College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, P. R. China, 2Institute of Agricultural Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Science, Wuhan 430064, P. R. China |
Bibliography |
Journal of Microbiology, 56(4),255–263, 2018,
|
DOI |
10.1007/s12275-018-7425-8
|
Key Words |
Monasucs ruber, comparative proteomics, development,
modulation, regulator of G protein α subunit, secondary
metabolites |
Abstract |
Monascus spp. are commonly used for a wide variety of applications
in the food and pharmaceutical industries. In previous
studies, the knock-out of mrflbA (a putative regulator
of the G protein α subunit) in M. ruber led to autolysis of
the mycelia, decreased pigmentation and lowered mycotoxin
production. Therefore, we aimed to obtain a comprehensive
overview of the underlying mechanism of mrflbA deletion
at the proteome level. A two-dimensional gel electrophoresis
analysis of mycelial proteins indicated that the abundance
of 178 proteins was altered in the ΔmrflbA strain, 33 of which
were identified with high confidence. The identified proteins
are involved in a range of activities, including carbohydrate
and amino acid metabolism, hyphal development and the oxidative
stress response, protein modification, and the regulation
of cell signaling. Consistent with these findings, the activity
of antioxidative enzymes and chitinase was elevated in
the supernatant of the ΔmrflbA strain. Furthermore, deletion
of mrflbA resulted in the transcriptional reduction of secondary
metabolites (pigment and mycotoxin). In short, the
mutant phenotypes induced by the deletion of mrflbA were
consistent with changes in the expression levels of associated
proteins, providing direct evidence of the regulatory functions
mediated by mrflbA in M. ruber. |