| Title | A murine colitis model developed using a combination of dextran sulfate sodium and Citrobacter rodentium |
|---|---|
| Author | Jin-Il Park1,2, Sun-Min Seo1, Jong-Hyung Park1, Hee-Yeon Jeon1,3, Jun-Young Kim1, Seung-Hyun Ryu1,4, and Yang-Kyu Choi1* |
| Address | 1Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea, 2ViroMed Co. Ltd., Seoul 08826, Republic of Korea, 3Department of Core Research Laboratory, Clinical Research Institute, Kyung Hee University Hospital at Gangdong, Seoul 05278, Republic of Korea, 4Samjin Pharmaceutical Co., Ltd., Seoul 04054, Republic of Korea |
| Bibliography | Journal of Microbiology, 56(4),272–279, 2018, |
| DOI | 10.1007/s12275-018-7504-x |
| Key Words | Citrobacter rodentium, colitis, dextran sulfate sodium |
| Abstract | Adult mice were treated with dextran sulfate sodium (DSS) and infected with Citrobacter rodentium for developing a novel murine colitis model. C57BL/6N mice (7-week-old) were divided into four groups. Each group composed of control, dextran sodium sulfate-treated (DSS), C. rodentiuminfected (CT), and DSS-treated and C. rodentium-infected (DSS-CT) mice. The DSS group was administered 1% DSS in drinking water for 7 days. The CT group was supplied with normal drinking water for 7 days and subsequently infected with C. rodentium via oral gavage. The DSS-CT group was supplied with 1% DSS in drinking water for 7 days and subsequently infected with C. rodentium via oral gavage. The mice were sacrificed 10 days after the induction of C. rodentium infection. The DSS-CT group displayed significantly shorter colon length, higher spleen to body weight ratio, and higher histopathological score compared to the other three groups. The mRNA expression levels of tumor necrosis factor (TNF)-α and interferon (INF)-γ were significantly upregulated; however, those of interleukin (IL)-6 and IL-10 were significantly downregulated in the DSS-CT group than in the control group. These results demonstrated that a combination of low DSS concentration (1%) and C. rodentium infection could effectively induce inflammatory bowel disease (IBD) in mice. This may potentially be used as a novel IBD model, in which colitis is induced in mice by the combination of a chemical and a pathogen. |