| Title | Comparative genomic analysis of Geosporobacter ferrireducens and its versatility of anaerobic energy metabolism |
|---|---|
| Author | Man-Young Jung1, So-Jeong Kim2*, Jong-Geol Kim3, Heeji Hong3, Joo-Han Gwak3, Soo-Je Park4, Yang-Hoon Kim3, and Sung-Keun Rhee3* |
| Address | 1Department of Microbiology and Ecosystem Science, Division of Microbial Ecology, University of Vienna, A-1090 Vienna, Austria, 2Geologic Environment Research Division, Korea Institute of Geoscience and Mineral Resources, Daejeon 34132, Republic of Korea, 3Department of Microbiology, Chungbuk National University, Cheongju 28644, Republic of Korea, 4Department of Biology, Jeju National University, Jeju 63243, Republic of Korea |
| Bibliography | Journal of Microbiology, 56(5),365–371, 2018, |
| DOI | 10.1007/s12275-018-7451-6 |
| Key Words | Geosporobacter ferrireducens IRF9, Geosporobacter subterraneus DSM 17957, comparative genomics, ATPase, Rnf complex, toluene |
| Abstract | Members of the family Clostridiaceae within phylum Firmicutes are ubiquitous in various iron-reducing environments. However, genomic data on iron-reducing bacteria of the family Clostridiaceae, particularly regarding their environmental distribution, are limited. Here, we report the analysis and comparison of the genomic properties of Geosporobacter ferrireducens IRF9, a strict anaerobe that ferments sugars and degrades toluene under iron-reducing conditions, with those of the closely related species, Geosporobacter subterraneus DSM 17957. Putative alkyl succinate synthase-encoding genes were observed in the genome of strain IRF9 instead of the typical benzyl succinate synthase-encoding genes. Canonical genes associated with iron reduction were not observed in either genome. The genomes of strains IRF9 and DMS 17957 harbored genes for acetogenesis, that encode two types of Rnf complexes mediating the translocation of H+ and Na+ ions, respectively. Strain IRF9 harbored two different types of ATPases (Na+-dependent F-type ATPase and H+- dependent V-type ATPase), which enable full exploitation of ion gradients. The versatile energy conservation potential of strain IRF9 promotes its survival in various environmental conditions. |