Title |
Comparative genomic analysis of Geosporobacter ferrireducens and its versatility of anaerobic energy metabolism |
Author |
Man-Young Jung1, So-Jeong Kim2*, Jong-Geol Kim3, Heeji Hong3, Joo-Han Gwak3, Soo-Je Park4, Yang-Hoon Kim3, and Sung-Keun Rhee3* |
Address |
1Department of Microbiology and Ecosystem Science, Division of Microbial Ecology, University of Vienna, A-1090 Vienna, Austria, 2Geologic Environment Research Division, Korea Institute of Geoscience and Mineral Resources, Daejeon 34132, Republic of Korea, 3Department of Microbiology, Chungbuk National University, Cheongju 28644, Republic of Korea, 4Department of Biology, Jeju National University, Jeju 63243, Republic of Korea |
Bibliography |
Journal of Microbiology, 56(5),365–371, 2018,
|
DOI |
10.1007/s12275-018-7451-6
|
Key Words |
Geosporobacter ferrireducens IRF9, Geosporobacter
subterraneus DSM 17957, comparative genomics, ATPase,
Rnf complex, toluene |
Abstract |
Members of the family Clostridiaceae within phylum Firmicutes
are ubiquitous in various iron-reducing environments.
However, genomic data on iron-reducing bacteria of the family
Clostridiaceae, particularly regarding their environmental
distribution, are limited. Here, we report the analysis and
comparison of the genomic properties of Geosporobacter
ferrireducens IRF9, a strict anaerobe that ferments sugars
and degrades toluene under iron-reducing conditions, with
those of the closely related species, Geosporobacter subterraneus
DSM 17957. Putative alkyl succinate synthase-encoding
genes were observed in the genome of strain IRF9 instead
of the typical benzyl succinate synthase-encoding genes.
Canonical genes associated with iron reduction were not
observed in either genome. The genomes of strains IRF9 and
DMS 17957 harbored genes for acetogenesis, that encode two
types of Rnf complexes mediating the translocation of H+
and Na+ ions, respectively. Strain IRF9 harbored two different
types of ATPases (Na+-dependent F-type ATPase and H+-
dependent V-type ATPase), which enable full exploitation
of ion gradients. The versatile energy conservation potential
of strain IRF9 promotes its survival in various environmental
conditions. |