Title |
Core gut microbiota in Jinhua pigs and its correlation with strain, farm and weaning age |
Author |
Hua Yang1,2,3, Yingping Xiao2, Junjun Wang1,3, Yun Xiang4, Yujie Gong2, Xueting Wen2, and Defa Li1,3* |
Address |
1State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, P. R. China, 2Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P. R. China, 3Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing 100193, P. R. China, 4Institute of Animal Husbandry and Veterinary Medicine, Jinhua Academy of Agricultural Sciences, Jinhua 321000, P. R. China |
Bibliography |
Journal of Microbiology, 56(5),346–355, 2018,
|
DOI |
10.1007/s12275-018-7486-8
|
Key Words |
Jinhua pigs, core gut microbiota, short-chain
fatty acids, strain, farm, weaning age |
Abstract |
Gut microbial diversity and the core microbiota of the Jinhua
pig, which is a traditional, slow-growing Chinese breed with
a high body-fat content, were examined from a total of 105
fecal samples collected from 6 groups of pigs at 3 weaning
ages that originated from 2 strains and were raised on 3 different
pig farms. The bacterial community was analyzed following
high-throughput pyrosequencing of 16S rRNA genes,
and the fecal concentrations of short-chain fatty acids (SCFAs)
were measured by gas chromatograph. Our results showed
that Firmicutes and Bacteroidetes were the dominant phyla,
and Lactobacillus, Streptococcus, Clostridium, SMB53, and
Bifidobacterium were the most abundant genera. Fifteen predominant
genera present in every Jinhua pig sample constituted
a phylogenetic core microbiota and included the probiotics
Lactobacillus and Bifidobacterium, and the SCFAproducing
bacteria Clostridium, Prevotella, Bacteroides, Coprococcus,
Roseburia, Ruminococcus, Blautia, and Butyricicoccus.
Comparisons of the microbiota compositions and
SCFA concentrations across the 6 groups of pigs demonstrated
that genetic background and weaning age affected the
structure of the gut microbiota more significantly than the
farm. The relative abundance of the core genera in the pigs,
including Lactobacillus, Clostridium, Prevotella, Bacteroides,
Roseburia, Ruminococcus, Blautia, and Butyricicoccus varied
dramatically in pigs among the 2 origins and 3 weaning
ages, while Oscillospira, Megasphaera, Parabacteroides, and
Corynebacterium differed among pigs from different farms.
Interestingly, there was a more significant influence of strain
and weaning age than of rearing farm on the SCFA concentrations.
Therefore, strain and weaning age appear to be the
more important factors shaping the intestinal microbiome
of pigs. |