| Title | Highly diverse endophytes in roots of Cycas bifida (Cycadaceae), an ancient but endangered gymnosperm |
|---|---|
| Author | Ying Zheng1,2,3, Tzen-Yuh Chiang4, Chao-Li Huang4, and Xun Gong1,2* |
| Address | 1Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, P. R. China, 2Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, P. R. China, 3University of Chinese Academy of Sciences, Beijing 100049, P. R. China, 4Department of Life Sciences, National Cheng Kung University, Tainan 701, Taiwan |
| Bibliography | Journal of Microbiology, 56(5),337–345, 2018, |
| DOI | 10.1007/s12275-018-7438-3 |
| Key Words | coralloid root, cyanobacteria, Cycas, diversity, endophyte, next-generation sequencing technique |
| Abstract | As an ancient seed plant, cycads are one of the few gymnosperms that develop a root symbiosis with cyanobacteria, which has allowed cycads to cope with harsh geologic and climatic conditions during the evolutionary process. However, the endophytic microbes in cycad roots remain poorly identified. In this study, using next-generation sequencing techniques, we investigated the microbial diversity and composition of both the coralloid and regular roots of Cycas bifida (Dyer) K.D. Hill. Highly diverse endophytic communities were observed in both the coralloid and regular roots. Of the associated bacteria, the top five families were the Nostocaceae, Sinobacteraceae, Bradyrhizobiaceae, Bacillaceae, and Hyphomicrobiaceae. The Nectriaceae, Trichocomaceae, and Incertae sedis were the predominant fungal families in all root samples. A significant difference in the endophytic bacterial community was detected between coralloid roots and regular roots, but no difference was observed between the fungal communities in the two root types. Cyanobacteria were more dominant in coralloid roots than in regular roots. The divergence of cycad root structures and the modified physiological processes may have contributed to the abundance of cyanobionts in coralloid roots. Consequently, the colonization of cyanobacteria inhibits the assemblage of other endophytes. Our results contribute to an understanding of the species diversity and composition of the cycad-endophyte microbiome and provide an abbreviated list of potential ecological roles of the core microbes present. |