Title |
Characterization of Flavobacterium aquimarinum sp. nov., a halotolerant bacterium isolated from seawater |
Author |
Sylvia Kristyanto1, Tuan Manh Nguyen1,2, Dhiraj Kumar Chaudhary1, Sang-Seob Lee1, and Jaisoo Kim1* |
Address |
1Department of Life Science, College of Natural Sciences and Engineering, Kyonggi University, Suwon 16227, Republic of Korea, 2Thai Nguyen University of Agriculture and Forestry, Quyet Thang, Thai Nguyen, Vietnam |
Bibliography |
Journal of Microbiology, 56(5),317–323, 2018,
|
DOI |
10.1007/s12275-018-7454-3
|
Key Words |
Flavobacterium aquimarinum sp. nov., seawater,
halotolerant, Flavobacteriaceae, bacteroidetes |
Abstract |
A novel, aerobic, Gram-stain-negative, non-motile, non-spore
forming, rod-shaped bacterium, designated strain Dol 15-39T,
was isolated from a seawater sample near Geoje Island in
the South Sea, Republic of Korea. The strain was found to
be oxidase-negative and catalase-positive. The isolate was
observed to grow at temperatures from 4 to 37°C, at salinities
of up to 7%, and at pH levels from 6 to 9; moreover, it
was not able to degrade starch, DNA, esculin, or tyrosine.
Phylogenetic analysis based on 16S rRNA gene sequences
showed that Dol 15-39T was most closely related to Flavobacterium
jumunjinense HME7102T with a sequence similarity
of 97.3%. However, the levels of DNA-DNA relatedness
between Dol 15-39T and the most closely related species were
much lower than 70%, confirming that they represented distinct
genomic species. The genomic DNA G + C content of
Dol 15-39T was calculated to be 32.6 mol%. MK-6 was the
predominant respiratory quinine, while iso-C15:0 (25.0%), iso-
C15:1 G (17.0%), and iso-C17:0 3-OH (10.4%) were the major
cellular fatty acids. Phosphatidylethanolamine was identified
as a major polar lipid, while various unidentified aminolipids
and polar lipids were also detected. Based on polyphasic
taxonomic data, Dol 15-39T represents a novel species
of the genus Flavobacterium, for which the name F. aquimarinum
sp. nov. is proposed. The type strain is accessible
under the culture collection numbers (KEMB 9005-617T =
JCM 31930T). |