Title |
Leifsonia flava sp. nov., a novel actinobacterium isolated from the rhizosphere of Aquilegia viridiflora |
Author |
Ying Cai, Wen-Zhen Tao, Yu-Jun Ma, Juan Cheng, Meng-Yue Zhang, and Yi-Xuan Zhang* |
Address |
School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, 110016, P. R. China |
Bibliography |
Journal of Microbiology, 56(8),549–555, 2018,
|
DOI |
10.1007/s12275-018-8061-z
|
Key Words |
Leifsonia flava sp. nov., Gram-positive, rhizosphere,
Aquilegia viridiflora, polyphasic taxonomy |
Abstract |
SYP-B2174T is a yellow-pigmented, Gram-positive, non-motile,
and rod-shaped actinobacterium isolated from the rhizospheric
soil of Aquilegia viridiflora Pall. collected from the
Xinjiang uygur autonomous region of China. The strain’s
growth temperature ranges from 1 to 35°C, with an optimal
growth being observed at 28°C. Growth occurs from 0 to 5%
NaCl and at pH 6–8, with optimal growth being observed
in 1% NaCl at pH 7. Comparative 16S rRNA gene sequencebased
phylogenetic analysis placed the strain in a clade with
the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans
DSM 22824T with similarities of 97.8 and 97.6%,
respectively. The DNA-DNA hybridization values of the strain
SYP-B2174T to its closest phylogenetic neighbors were significantly
lower than 35.7%. The strain was identified as a novel
species of the genus Leifsonia judging by the coryneform morphology,
peptidoglycans based upon 2,4-diaminobutyric acid,
principal phospholipids phosphatidylglycerol and diphosphatidylglycerol,
major menaquinone MK-11, predominant
fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a
DNA G + C base composition of 68.7 mol%, for which the
name Leifsonia flava sp. nov. is proposed. The type strain is
SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC
39963T). |