| Title | Diet-induced obese mice exhibit altered immune responses to early Salmonella Typhimurium oral infection |
|---|---|
| Author | Ricardo Ernesto Ramírez-Orozco1, Elena Franco Robles2, Victoriano Pérez Vázquez3, Joel Ramírez Emiliano3, Marco Antonio Hernández Luna4, and Sergio López Briones4* |
| Address | 1Department of Clinical Nutrition, Autonomous University of Aguascalientes, Aguascalientes 20131, México, 2Department of Agronomy, Division of Life Sciences, Campus Irapuato Salamanca, University of Guanajuato, 36500, México, 3Department of Medical Sciences, Division of Health Sciences, Campus León, University of Guanajuato, 37000, México, 4Department of Medicine and Nutrition, Division of Health Sciences, Campus León, University of Guanajuato, 37670, México |
| Bibliography | Journal of Microbiology, 56(9),673–682, 2018, |
| DOI | 10.1007/s12275-018-8083-6 |
| Key Words | obesity, Sallmonella Typhimurium, HFD, immune response |
| Abstract | Obesity is a chronic disease associated with different metabolic diseases as well as alterations in immune cell function. It is characterized by a chronic systemic low grade inflammation. There are several studies demonstrating the influence of obesity on the impaired immune response to infection. However, it is not completely clear whether the obese environment influences the development or maintenance of the immune response against infections. The aim of this study was to determine how obesity induced by a high-fat diet affects the immune response to an early oral Salmonella infection. Four groups of mice were kept in separate cages. Two of these designated as controls, fed with a normal diet; whereas other two groups were fed with a high fat diet for 10 weeks. Some mice were used for Salmonella oral infection. After 7 days of oral infection with S. Thypimurium the proportions of spleen cell subsets expressing activation markers in normal diet and HFD obese mice were stained with monoclonal antibodies and analyzed by flow cytometry. Also, mRNA levels of different cytokines were quantified by RT-PCR. It was found that obesity affects the function of the immune system against an early oral Salmonella infection, decreasing NK cells, altering the expression of activation molecules as well as cytokines mRNA levels. Interestingly, the expression some activation molecules on T lymphocytes was reestablished after Salmonella infection, but not the CD25 expression. Immune alterations could lead to immunosuppression or increased susceptibility to infections in HFD obese mice. |