Title |
Streptomyces sp. strain SK68, isolated from peanut rhizosphere, promotes growth and alleviates salt stress in tomato (Solanum lycopersicum cv. Micro-Tom) |
Author |
Karthiyaini Damodharan1, Sasikumar Arunachalam Palaniyandi2, Bao Le3, Joo-Won Suh1*, and Seung Hwan Yang3* |
Address |
1Center for Nutraceutical and Pharmaceutical Materials, College of Natural Science, Myongji University, Yongin 17058, Republic of Korea, 2Department of Biotechnology, Mepco Schlenk Engineering College, Mepco Nagar, Mepco Engineering College Post-626005, Sivakasi, Tamilnadu, India, 3Department of Biotechnology, Chonnam National University, Yeosu 59626, Republic of Korea |
Bibliography |
Journal of Microbiology, 56(10),753–759, 2018,
|
DOI |
10.1007/s12275-018-8120-5
|
Key Words |
plant growth promotion, Streptomyces, salt-stress,
tomato |
Abstract |
A novel actinobacterium, strain SK68, was isolated from the
rhizosphere of peanut plant and its salinity stress alleviation
ability was studied using tomato (Solanum lycopersicum
cv. Micro-Tom) plants. Based on 16S rDNA based phylogenetic
analysis, strain SK68 has been identified as a Streptomyces
sp. Strain SK68 had branched substrate mycelium bearing
smooth surfaced spores and the spore colour is brownish
grey on ISP4 medium. It exhibited enzyme activities such
as xylanase, cellulase, amylase, and pectinase and degraded
hypoxanthine, casein, and L-tyrosine. The strain SK68 differed
in its banding pattern in BOX-PCR and RAPD fingerprinting
compared to the closely matching type strains
Streptomyces erythrochromogenes NBRC 3304T (AB184746),
S. flavotricini NBRC 12770T (AB184132), S. racemochromogenes
NBRC 12906T (AB184235), and S. polychromogenes
NBRC 13072T (NR041109). Strain SK68 was evaluated for
its salinity stress-alleviating activity in tomato plants with
180 mmol/L NaCl under gnotobiotic condition. A significant
increase in plant biomass was observed in strain SK68-inoculated
tomato plants under salt stress compared to control
and salt-stressed non-inoculated plants. |