| Title | Brevibacterium anseongense sp. nov., isolated from soil of ginseng field |
|---|---|
| Author | Mi-Seon Jung1, Xiao-Tian Quan2, Muhammad Zubair Siddiqi1, Qingzhen Liu1, Sang Yong Kim3, Ji-Hyang Wee3, and Wan Taek Im1,4* |
| Address | 1Department of Biotechnology, Hankyong National University, Anseong 17579, Republic of Korea, 2Department of Biological Sciences, School of Life Sciences, Nantong University, Jiangsu, 226-019, P. R. China, 3Department of Food Science & Bio Technology, Shinansan University, Ansan 15435, Republic of Korea, 4AceEMzyme Co., Ltd., Anseong 17579, Republic of Korea |
| Bibliography | Journal of Microbiology, 56(10),706–712, 2018, |
| DOI | 10.1007/s12275-018-8181-5 |
| Key Words | Brevibacterium anseongensis, 16S rRNA gene sequence, polyphasic taxonomy |
| Abstract | A Gram-positive, aerobic, non-motile, pale-yellow, and rodshaped bacterium, designated as Gsoil 188T, was isolated from the soil of a ginseng field in Pocheon, South Korea. A phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to B. epidermidis NBRC 14811T (98.4%), B. sediminis FXJ8.269T (98.2%), B. avium NCFB 3055T (98.1%), and B. oceani BBH7T (98.1%), while it shared less than 98.1% identity with the other species of this genus. The DNA G + C content was 68.1 mol%. The predominant quinone was MK-8(H2). The major fatty acids were anteiso-C15:0 and anteiso-C17:0. The cell wall peptidoglycan of strain Gsoil 188T contained meso-diaminopimelic acid. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, and an unidentified aminolipid. The physiological and biochemical characteristics, low DNA-DNA relatedness values, and taxonomic analysis allowed the differentiation of strain Gsoil 188T from the other recognized species of the genus Brevibacterium. Therefore, strain Gsoil 188T represents a novel species of the genus Brevibacterium, for which the name Brevibacterium anseongense sp. nov. is proposed, with the type strain Gsoil 188T (= KACC 19439T = LMG 30331T). |