Title |
[MINIREVIEW] Progress of analytical tools and techniques for human gut microbiome research |
Author |
Eun-Ji Song1,2, Eun-Sook Lee1, and Young-Do Nam1,2* |
Address |
1Research Group of Healthcare, Korea Food Research Institute, Wanju 55365, Republic of Korea, 2Department of Food Biotechnology, Korea University of Science and Technology, Daejeon 34113, Republic of Korea |
Bibliography |
Journal of Microbiology, 56(10),693–705, 2018,
|
DOI |
10.1007/s12275-018-8238-5
|
Key Words |
gut microbiota, microbiome, NGS, bioinformatics,
analytical process |
Abstract |
Massive DNA sequencing studies have expanded our insights
and understanding of the ecological and functional
characteristics of the gut microbiome. Advanced sequencing
technologies allow us to understand the close association of
the gut microbiome with human health and critical illnesses.
In the future, analyses of the gut microbiome will provide
key information associating with human individual health,
which will help provide personalized health care for diseases.
Numerous molecular biological analysis tools have been rapidly
developed and employed for the gut microbiome researches;
however, methodological differences among researchers
lead to inconsistent data, limiting extensive share of
data. It is therefore very essential to standardize the current
methodologies and establish appropriate pipelines for human
gut microbiome research. Herein, we review the methods
and procedures currently available for studying the human
gut microbiome, including fecal sample collection, metagenomic
DNA extraction, massive DNA sequencing, and data
analyses with bioinformatics. We believe that this review will
contribute to the progress of gut microbiome research in
the clinical and practical aspects of human health. |