| Title | Mediterraneibacter butyricigenes sp. nov., a butyrate-producing bacterium isolated from human faeces |
|---|---|
| Author | Ji-Sun Kim1, Keun Chul Lee1, Min Kuk Suh1, Kook-Il Han1, Mi Kyung Eom1, Ju Huck Lee1, Seung-Hwan Park1, Se Won Kang1, Jam-Eon Park1, Byeong Seob Oh1, Seung Yeob Yu1, Seung-Hyeon Choi1, Dong Ho Lee2, Hyuk Yoon2, Byung-Yong Kim3, Seung-Jo Yang3, and Jung-Sook Lee1,4 |
| Address | 1Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea, 2Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea, 3ChunLab Inc., Seoul 06725, Republic of Korea, 4University of Science and Technology, Daejeon 34113, Republic of Korea |
| Bibliography | Journal of Microbiology, 57(1),38–44, 2019, |
| DOI | 10.1007/s12275-019-8550-8 |
| Key Words | Mediterraneibacter butyricigenes sp. nov., butyrate, human faeces |
| Abstract | A Gram-stain-positive, obligately anaerobic, non-motile, nonspore- forming, and rod-shaped bacterial strain, designated KGMB01110T, was isolated from a faecal sample of a healthy male in South Korea. Phylogenetic analysis based on 16S rRNA gene showed that strain KGMB01110T belonged to Clostridium cluster XIVa and was most closely related to Mediterraneibacter glycyrrhizinilyticus KCTC 5760T (95.9% 16S rRNA gene sequence similarity). The DNA G + C content of strain KGMB01110T based on its whole genome sequence was 44.1 mol%. The major cellular fatty acids (> 10%) of the isolate were C14:0 and C16:0. The strain KGMB01110T was positive for arginine dihydrolase, β-galactosidase-6-phosphatase, and alkaline phosphatase. The strain KGMB01110T also produced acid from D-glucose and D-rhamnose, and hydrolyzed gelatin and aesculin. Furthermore, HPLC analysis and UV-tests of culture supernatant revealed that the strain KGMB01110T produced butyrate as the major end product of glucose fermentation. Based on the phylogenetic and phenotypic characteristics, strain KGMB01110T represent a novel species of the genus Mediterraneibacter in the family Lachnospiraceae. The type strain is KGMB01110T (= KCTC 15684T = CCUG 72830T). |