Title |
Lipoteichoic acids of lactobacilli inhibit Enterococcus faecalis biofilm formation and disrupt the preformed biofilm |
Author |
Solmin Jung1, Ok-Jin Park1, A Reum Kim1, Ki Bum Ahn1,2, Dongwook Lee1, Kee-Yeon Kum3, Cheol-Heui Yun4,5, and Seung Hyun Han1* |
Address |
1Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea, 2Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea, 3Department of Conservative Dentistry, DRI, and Seoul Dental Hospital for Disabled, School of Dentistry, Seoul National University, Seoul 03080, Republic of Korea, 4Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea, 5Institute of Green Bio Science Technology, Seoul National University, Pyeongchang 25354, Republic of Korea |
Bibliography |
Journal of Microbiology, 57(4),310–315, 2019,
|
DOI |
10.1007/s12275-019-8538-4
|
Key Words |
biofilm, Enterococcus faecalis, lipoteichoic acid,
lactobacilli, apical periodontitis |
Abstract |
Enterococcus faecalis, a Gram-positive bacterium commonly
isolated in patients with refractory apical periodontitis, invades
dentin tubules easily and forms biofilms. Bacteria in biofilms,
which contribute to recurrent and/or chronic inflammatory
diseases, are more resistant to antimicrobial agents
than planktonic cells and easily avoid phagocytosis. Although
Lactobacillus plantarum lipoteichoic acid (Lp.LTA) is associated
with biofilm formation, the effect of Lp.LTA on biofilm
formation by E. faecalis is not clearly understood. In this
study, we investigated whether Lp.LTA inhibits E. faecalis
biofilm formation. The degree of biofilm formation was determined
by using crystal violet assay and LIVE/DEAD bacteria
staining. The quantification of bacterial growth was determined
by measuring the optical density at 600 nm with a
spectrophotometer. Formation of biofilms on human dentin
slices was observed under a scanning electron microscope.
E. faecalis biofilm formation was reduced by Lp.LTA treatment
in a dose-dependent manner. Lp.LTA inhibited biofilm
development of E. faecalis at the early stage without affecting
bacterial growth. LTA from other Lactobacillus species
such as Lactobacillus acidophilus, Lactobacillus casei, or
Lactobacillus rhamnosus GG also inhibited E. faecalis biofilm
formation. In particular, among LTAs from various lactobacilli,
Lp.LTA showed the highest inhibitory effect on biofilms
formed by E. faecalis. Interestingly, LTAs from lactobacilli
could remove the biofilm preformed by E. faecalis.
These inhibitory effects were also observed on the surface of human dentin slices. In conclusion, Lactobacillus species LTA
inhibits biofilm formation caused by E. faecalis and it could
be used as an anti-biofilm agent for prevention or treatment
against E. faecalis-associated diseases. |