Title Mesorhizobium denitrificans sp. nov., a novel denitrifying bacterium isolated from sludge
Author Muhammad Zubair Siddiqi1,2, Ngo Thi Phuong Thao3, Gyumin Choi2, Dae-Cheol Kim1, Young-Woo Lee1, Sang Young Kim4, Ji-Hyang Wee4, and Wan-Taek Im1,2*
Address 1Department of Biotechnology, Hankyong National University, Anseong 17579, Republic of Korea, 2AceEMzyme Co., Ltd., Academic Industry Cooperation, Anseong 17579, Republic of Korea, 3M-ChET, KIOCA program, Hankyong National University, Anseong 17579, Republic of Korea, 4Department of Food Science & Bio Technology, Shinansan University, Ansan 15435, Republic of Korea
Bibliography Journal of Microbiology, 57(4),238–242, 2019,
DOI 10.1007/s12275-019-8590-0
Key Words Mesorhizobium denitrificans, 16S rRNA gene sequence, polyphasic taxomony, sludge
Abstract A Gram-stain-negative, non-spore-forming, facultative, rodshaped bacterium (designated LA-28T) was isolated from a sludge sample from a wastewater treatment plant in Hanam city, Republic of Korea. On the basis of 16S rRNA gene sequencing, strain LA-28T clustered with species of the genus Mesorhizobium and appeared closely related to M. jarvisii LMG 28313T (96.8%), M. waimense ICMP 19557T (96.7%), and M. huakuii LMG 14107T (96.7%). Growth occurs at 18– 40°C on R2A medium in the presence of 1–4% NaCl (w/v) and at pH 6–8. The DNA G+C content was 61.2 mol%, and the predominant quinone was ubiquinone-10 (Q-10). The major cellular fatty acids (> 5%) were C16:0, C19:0 ω8c cyclo, C18:1 ω7c 11-methyl, and C18:1 ω7c and/or C18:1 ω6c (summed feature 8). Major polar lipids were phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidyl-N-methylethanolamine (PME), and phosphatidylcholine (PC). Physiological and biochemical characteristics indicated that strain LA-28T represents a novel species of the genus Mesorhizobium, for which the name Mesorhizobium denitrificans sp. nov. is proposed. The type strain is LA-28T (= KACC 19675T = LMG 30806T).