Title |
Biocontrol activity of volatile organic compounds from Streptomyces alboflavus TD-1 against Aspergillus flavus growth and aflatoxin production |
Author |
Mingguan Yang, Laifeng Lu, Jing Pang, Yiling Hu, Qingbin Guo, Zhenjing Li, Shufen Wu, Huanhuan Liu, and Changlu Wang* |
Address |
State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, P. R. China |
Bibliography |
Journal of Microbiology, 57(5),396–404, 2019,
|
DOI |
10.1007/s12275-019-8517-9
|
Key Words |
volatile organic compounds, biocontrol, Streptomyces
alboflavus TD-1, Aspergillus flavus, aflatoxin B1 |
Abstract |
Aspergillus flavus is a saprophytic fungus that contaminates
crops with carcinogenic aflatoxin. In the present work, the
antifungal effects of volatile organic compounds (VOCs) from
Streptomyces alboflavus TD-1 against A. flavus were investigated.
VOCs from 8-day-old wheat bran culture of S. alboflavus
TD-1 displayed strong inhibitory effects against mycelial
growth, sporulation, and conidial germination of A.
flavus. Severely misshapen conidia and hyphae of A. flavus
were observed by scanning electron microscopy after exposure
to VOCs for 6 and 12 h, respectively. Rhodamine 123
staining of mitochondria indicated that mitochondria may
be a legitimate antifungal target of the VOCs from S. alboflavus
TD-1. Furthermore, the VOCs effectively inhibited
aflatoxin B1 production by downregulating genes involved
in aflatoxin biosynthesis. Dimethyl trisulfide and benzenamine
may play important roles in the suppression of A. flavus
growth and production of aflatoxin. The results indicate
that VOCs from S. alboflavus TD-1 have tremendous potential
to be developed as a useful bio-pesticide for controlling
A. flavus. |