Title |
Flavobacterium aquariorum sp. nov., isolated from freshwater of the North Han River |
Author |
Yochan Joung, Hye-Jin Jang, Jaeho Song, and Jang-Cheon Cho* |
Address |
Department of Biological Sciences, Inha University, Incheon 22212, Republic of Korea |
Bibliography |
Journal of Microbiology, 57(5),343–349, 2019,
|
DOI |
10.1007/s12275-019-8436-9
|
Key Words |
Flavobacterium, F. aquariosum, Han River, taxonomy,
genome, bacteria |
Abstract |
A non-motile, yellow-pigmented bacterial strain, designated
IMCC34762T, was isolated from a freshwater sample collected
from Lake Cheongpyeong in Korea. Phylogenetic analysis
based on 16S rRNA gene sequences showed that strain IMCC-
34762T formed a lineage within the genus Flavobacterium
and was most closely related to F. pectinovorum DSM 6368T
(98.3% sequence similarity), followed by F. piscis CCUG
60099T (98.3%), F. branchiicola 59B-3-09T (98.2%), and F.
saccharophilum DSM 1811T (98.2%). The average nucleotide
identity and the genome-to-genome distance between strain
IMCC34762T and the closely related strains were 61–62%
and 26–27%, respectively, indicating that IMCC34762T is a
novel species of the genus Flavobacterium. The major fatty
acids (> 5%) of strain IMCC34762T were summed feature 3
(C16:1 ω6c and/or C16:1 ω7c, 17.3%), iso-C15:0 (15.0%), iso-C15:0
G (9.0%), C15:0 ω6c (7.4%), iso-C15:0 (7.4%), and iso-C16:0 (5.3%).
The major respiratory quinone and polyamine were MK-6 and
sym-homospermidine, respectively. The major polar lipids
were phosphatidylethanolamine, an unidentified aminophospholipid,
and an unidentified lipid. The DNA G+C content
of strain IMCC34762T was 34.4 mol%. Based on the taxonomic
data presented in this study, strain IMCC34762T represents
a novel species within the genus Flavobacterium, for which
the name Flavobacterium aquariorum, sp. nov. is proposed.
The type strain is IMCC34762T (= KACC 19725T = NBRC
113425T). |