| Title | Two novel synthetic peptides inhibit quorum sensing-dependent biofilm formation and some virulence factors in Pseudomonas aeruginosa PAO1 |
|---|---|
| Author | Mostafa N. Taha1*, Amal E. Saafan2, A. Ahmedy3, Eman El Gebaly4, and Ahmed S. Khairalla4 |
| Address | 1Department of Microbiology and Immunology, Faculty of Pharmacy, Nahda University, Beni-Suef, Egypt, 2Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Menoufia University, Menoufia, Egypt, 3Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt, 4Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt |
| Bibliography | Journal of Microbiology, 57(7),618–625, 2019, |
| DOI | 10.1007/s12275-019-8548-2 |
| Key Words | biofilm, peptides, quorum sensing, virulence factors |
| Abstract | Quorum sensing (QS) regulates virulence factor expression in Pseudomonas aeruginosa. Inhibiting the QS-controlled virulence factors without inhibiting the growth of P. aeruginosa is a promising approach for overcoming the widespread resistance of P. aeruginosa. This study was proposed to investigate the effects of two novel synthetic peptides on the biofilm development and virulence factor production of P. aeruginosa. The tested strain was P. aeruginosa PAO1. The results indicated that both of the synthetic peptides (LIVRHK and LIVRRK) inhibited (P < 0.05) the formation of biofilms and the production of virulence factors, including pyocyanin, protease, and rhamnolipids, without inhibiting the growth of PAO1. Additionally, we detected transcriptional changes related to QS and found a significant reduction in the levels of gene expression of lasI, lasR, rhlI, and rhlR. This study demonstrates that LIVRRK and LIVRHK are novel synthetic peptides that can act as potent inhibitors of QS-regulated virulence factors in P. aeruginosa. Moreover, these synthetic peptides have potential applications in the treatment of biofilmrelated diseases. Both peptides may be able to control chronic infections and biofilm-associated problems of P. aeruginosa. |