Title |
Two novel synthetic peptides inhibit quorum sensing-dependent biofilm formation and some virulence factors in Pseudomonas aeruginosa PAO1 |
Author |
Mostafa N. Taha1*, Amal E. Saafan2, A. Ahmedy3, Eman El Gebaly4, and Ahmed S. Khairalla4 |
Address |
1Department of Microbiology and Immunology, Faculty of Pharmacy, Nahda University, Beni-Suef, Egypt, 2Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Menoufia University, Menoufia, Egypt, 3Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt, 4Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt |
Bibliography |
Journal of Microbiology, 57(7),618–625, 2019,
|
DOI |
10.1007/s12275-019-8548-2
|
Key Words |
biofilm, peptides, quorum sensing, virulence factors |
Abstract |
Quorum sensing (QS) regulates virulence factor expression
in Pseudomonas aeruginosa. Inhibiting the QS-controlled virulence
factors without inhibiting the growth of P. aeruginosa
is a promising approach for overcoming the widespread
resistance of P. aeruginosa. This study was proposed to investigate
the effects of two novel synthetic peptides on the biofilm
development and virulence factor production of P. aeruginosa.
The tested strain was P. aeruginosa PAO1. The results
indicated that both of the synthetic peptides (LIVRHK and
LIVRRK) inhibited (P < 0.05) the formation of biofilms and
the production of virulence factors, including pyocyanin, protease,
and rhamnolipids, without inhibiting the growth of
PAO1. Additionally, we detected transcriptional changes related
to QS and found a significant reduction in the levels of
gene expression of lasI, lasR, rhlI, and rhlR. This study demonstrates
that LIVRRK and LIVRHK are novel synthetic peptides
that can act as potent inhibitors of QS-regulated virulence
factors in P. aeruginosa. Moreover, these synthetic peptides
have potential applications in the treatment of biofilmrelated
diseases. Both peptides may be able to control chronic
infections and biofilm-associated problems of P. aeruginosa. |