Title |
Evolution of a major bovine mastitic genotype (rpoB sequence type 10-2) of Staphylococcus aureus in cows |
Author |
Dae-Sung Ko1,2, Danil Kim1, Eun-Kyung Kim1, Jae-Hong Kim2,3, and Hyuk-Joon Kwon1,3* |
Address |
1Department of Farm Animal Medicine, Seoul National University, Seoul 08826, Republic of Korea, 2Laboratory of Avian Diseases, Seoul National University, Seoul 08826, Republic of Korea, 3The Research Institute for Veterinary Science, College of Veterinary, Medicine and BK21 for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea |
Bibliography |
Journal of Microbiology, 57(7),587–596, 2019,
|
DOI |
10.1007/s12275-019-8699-1
|
Key Words |
Staphylococcus aureus, bovine mastitis, comparative
genomics, pseudogenes, virulence genes |
Abstract |
Staphylococcus aureus is the major pathogen leading to bovine
mastitis globally while livestock-associated methicillin
resistant S. aureus (LA-MRSA) has become a potential threat
to public health. MRSA from bovine mastitis is not common
but a methicillin susceptible S. aureus (MSSA) genotype, rpoB
sequence type (RST)10-2 (RST10-2), is prevalent in Korea.
To date, many genomic sequences from S. aureus have been
elucidated, but the complete genome sequences of RST10-2
MSSA from bovine mastitis has never been reported. In this
study, we determined the complete genome sequence of two
RST10-2 MSSA that differ from each other in staphylococcal
protein A and molecular prophage types [PMB64-1 (t2489/
mPPT0) and PMB81-4 (t127/mPPT1-2-3)] and conducted
a comparative genomics study. The genomic sequences of
PMB64-1 and PMB81-4 were more homologous to the representative
human RST10-2 strains (MSSA476, MW2 etc.)
compared to other RSTs. Most of them shared five common
pseudogenes, along with high amino acid identity of four
variable virulence genes that were identified in this study.
However, PMB64-1 and PMB81-4 acquired different strainspecific
pseudogenes and mobile genetic elements than the
human strains. The unique pseudogene profile and high identity
of the virulence genes were verified in RST10-2 field strains
from bovine mastitis. Thus, bovine mastitic RST10-2 MSSA
may have an evolutionary relationship with the human RST10-
2 community-associated (CA) MSSA and CA-MRSA strains
but may have adapted to cows. |