| Title | Paenibacillus psychroresistens sp. nov., isolated from the soil of an Arctic glacial retreat |
|---|---|
| Author | In-Tae Cha1, Eui-Sang Cho2, Yoo Kyung Lee3, Seong Woon Roh1*, and Myung-Ji Seo2,4* |
| Address | 1Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea, 2Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University, Incheon 22012, Republic of Korea, 3Korea Polar Research Institute, Incheon 21990, Republic of Korea, 4Division of Bioengineering, Incheon National University, Incheon 22012, Republic of Korea |
| Bibliography | Journal of Microbiology, 57(7),569–574, 2019, |
| DOI | 10.1007/s12275-019-8666-x |
| Key Words | Paenibacillus psychroresistens, arctic, isolation, polyphasic taxonomy |
| Abstract | Strain ML311-T8T was isolated from a glacial retreat area in Svalbard, Norway, and was taxonomically characterized by a polyphasic approach. Upon phylogenetic analysis, strain ML311-T8T was clustered with Paenibacillus arcticus MME2_ R6T and P. contaminans CKOBP-6T with 98.3–98.6 and 93.5– 93.9% 16S rRNA gene sequence similarities, respectively. DNA-DNA hybridization values between strain ML311-T8T and P. arcticus MME2_R6T was 19.9%. The genomic DNA G+C content was 41.1 mol%. The isolated strain was Gramstain- positive, strictly aerobic and rod-shaped, and grew in 0–0.5% (w/v) NaCl, at 4–23°C and pH 6.0–10.0, with optimal growth in 0% (w/v) NaCl, at 20°C and pH 7.0–8.0. The predominant respiratory quinone of strain ML311-T8T was MK- 7 and the major fatty acids were anteiso-C15:0 and C16:0. The polar lipids of strain ML311-T8T were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, three unidentified amino lipids, and three unidentified lipids. On the basis of polyphasic taxonomic analysis, the strain ML311-T8T is proposed to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus psychroresistens sp. nov. is proposed. The type strain is ML311-T8T (= KCCM 43190T = JCM 31243T). |