Title |
Isolation and characterization of a novel piezotolerant bacterium Lysinibacillus yapensis sp. nov., from deep-sea sediment of the Yap Trench, Pacific Ocean |
Author |
Libo Yu1,2, Xixiang Tang2, Shiping Wei3, Yinkun Qiu4, Xiashutong Xu2,3, Guangxin Xu2,3, Qilin Wang2,3, and Qian Yang1* |
Address |
1School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150080, P. R. China, 2Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, P. R. China, 3School of Ocean Sciences, China University of Geosciences, Beijing 100083, P. R. China, 4Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, P. R. China |
Bibliography |
Journal of Microbiology, 57(7),562–568, 2019,
|
DOI |
10.1007/s12275-019-8709-3
|
Key Words |
Lysinibacillus yapensis sp. nov., deep-sea sediment,
Yap Trench |
Abstract |
A Gram-positive, aerobic, rod-shaped, spore-forming bacterium,
designated YLB-03T, with peritrichous flagella was
isolated from deep-sea sediment of the Yap Trench at a depth
of 4435 m. The bacterium was found to be catalase-positive
but oxidase-negative. Growth of this bacterium was observed
at 15–50°C (optimum 37°C), pH 5–10.5 (optimum 7), 0–5%
NaCl (optimum 1%, w/v) and 0.1–50 MPa (optimum 0.1
MPa). Phylogenetic analysis based on 16S rRNA gene sequences
showed that strain YLB-03T was a member of the
genus Lysinibacillus. Strain YLB-03T was closely related to
Lysinibacillus sinduriensis BLB-1T and Lysinibacillus chungkukjangi
2RL3-2T (98.4%), Lysinibacillus halotolerans LAM-
612T (98.0%), Lysinibacillus telephonicus KT735049T (97.5%),
Lysinibacillus endophyticus C9T (97.5%), Lysinibacillus composti
NCCP-36T and Lysinibacillus massiliensis 4400831T
(97.3%). The ANI and the GGDC DNA-DNA hybridization
estimate values between strain YLB-03T and closely related
type strains were 73.7–76.3% and 34.7–38.7%, respectively.
The principal fatty acids were anteiso-C15:0 and iso-C15:0. The
G+C content of the chromosomal DNA was 39.6 mol%. The
respiratory quinone was determined to be MK-7. The diagnostic
amino acids in the cell wall peptidoglycan contained
Lys-Asp (type A4α) and the cell-wall sugars were glucose
and xylose. The polar lipids included diphosphatidylglycerol,
phosphatidylglycerol, phosphatidylethanolamine, and an unidentified
phospholipid. The combined genotypic and phenotypic
data showed that strain YLB-03T represents a novel
species within the genus Lysinibacillus, for which the name
Lysinibacillus yapensis sp. nov. is proposed, with the type
strain YLB-03T (= MCCC 1A12698T = JCM 32871T). |