| Title | Isolation, cultivation, and genome analysis of proteorhodopsincontaining SAR116-clade strain Candidatus Puniceispirillum marinum IMCC1322 |
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| Author | Junhak Lee1,2, Kae Kyoung Kwon3, Seung-Il Lim4, Jaeho Song5, Ah Reum Choi6, Sung-Hyun Yang3, Kwang-Hwan Jung6, Jung-Hyun Lee3, Sung Gyun Kang3, Hyun-Myung Oh1*, and Jang-Cheon Cho5* |
| Address | 1Institute of Liberal Arts Education, Pukyong National University, Busan 48547, Republic of Korea, 2Korea Marine Equipment Research Institute, Busan 46754, Republic of Korea, 3Marine Biotechnology Research Division, Korea Institute of Ocean Science & Technology, Busan 49111, Republic of Korea, 4Cell Biotech, Co., Ltd., Gimpo 10003, Republic of Korea, 5Division of Biology and Ocean Sciences, Inha University, Incheon 22212, Republic of Korea, 6Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul 04107, Republic of Korea |
| Bibliography | Journal of Microbiology, 57(8),676–687, 2019, |
| DOI | 10.1007/s12275-019-9001-2 |
| Key Words | Canididatus Puniceispirillum marinum, carbon monoxide, dimethylsulfoniopropionate, light-emitting diode, mitomycin C, proteorhodopsin, SAR116 |
| Abstract | Strain IMCC1322 was isolated from a surface water sample from the East Sea of Korea. Based on 16S rRNA analysis, IMCC1322 was found to belong to the OCS28 sub-clade of SAR116. The cells appeared as short vibrioids in logarithmicphase culture, and elongated spirals during incubation with mitomycin or in aged culture. Growth characteristics of strain IMCC1322 were further evaluated based on genomic information; proteorhodopsin (PR), carbon monoxide dehydrogenase, and dimethylsulfoniopropionate (DMSP)-utilizing enzymes. IMCC1322 PR was characterized as a functional retinylidene protein that acts as a light-driven proton pump in the cytoplasmic membrane. However, the PR-dependent phototrophic potential of strain IMCC1322 was only observed under CO-inhibited and nutrient-limited culture conditions. A DMSP-enhanced growth response was observed in addition to cultures grown on C1 compounds like methanol, formate, and methane sulfonate. Strain IMCC1322 cultivation analysis revealed biogeochemical processes characteristic of the SAR116 group, a dominant member of the microbial community in euphotic regions of the ocean. The polyphasic taxonomy of strain IMCC1322 is given as Candidatus Puniceispirillum marinum, and was confirmed by chemotaxonomic tests, in addition to 16S rRNA phylogeny and cultivation analyses. |