Journal of Microbiology

Title A newly characterized exopolysaccharide from Sanghuangporus sanghuang
Author Xiao-kui Ma1*, Xu She1, Eric Charles Peterson2, Yu Zhen Wang1, Peng Zheng1, Hongyan Ma1, Kexin Zhang1, and Jian Liang1*
Address 1Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Science, Shaanxi Normal University, Xi’an 710055, Shaanxi, P. R. China, 2Agency for Science, Technology, and Research (A*STAR), 138673, Singapore
Bibliography Journal of Microbiology, 57(9),812–820, 2019,
DOI 10.1007/s12275-019-9036-4
Key Words Sanghuangporus sanghuang, biomacromolecule, exopolysaccharide, antioxidant, mammalian system
Abstract Sanghuangporus sanghuang is a well-known pharmacodynamic and economically important edible fungus associated with mulberry (Morus spp.). A distinctly new exopolysaccharide (EPS), designated SHP-2 was obtained from S. sanghuang P0988 broth, and its structure and anti-aging prosperity were characterized. SHP-2 was found to be composed of a backbone of 􎵔4)-β-Manp-(1􎵔4)-α-Araf-(1􎵔3,4)-α-Glcp(1􎵔􀁇 3,4)-α-Glcp-(1􎵔3,4)-α-Glcp-(1􎵔3,4)-α-Glcp-(1􎵔3,4)-α- Glcp-(1􎵔6)-α-Galp-(1􎵔4)-β-Manp-(1􎵔􀁇and five branches, including four α-D-Glcp-(1􎵔􀁇and one α-D-Manp-(1􎵔􀁇 SHP-2 was shown to increase antioxidant enzyme activities including catalase (CAT) and superoxide dismutase (SOD) activities, as well as trolox equivalent antioxidant (TEAC) capacity in serum of mice pre-treated with D-Gal, while reducing lipofuscin levels. SHP-2 exerted a favorable influence on immune organ coefficients and ameliorated the histopathological hepatic lesions and apoptosis in hepatocytes of Dgalactose- aged mice almost in a dose-dependent manner. Using the same analytical methods, on comparison with previously studied EPS compounds (i.e. SHP-1), SHP-2 was found to have more complex structure, larger molecule weight, and different anti-aging properties. The results presented here suggest that not only does EPS bioactivity vary with respect to molecular structures and molecule weight, but that multiple structures with different activity can be expressed by a single fungal strain. These results may help understanding the antiaging prosperity of these polysaccharides for use in health foods or dietary supplements.
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