Title |
Reduction of selenite to elemental Se(0) with simultaneous degradation of phenol by co-cultures of Phanerochaete chrysosporium and Delftia lacustris |
Author |
Samayita Chakraborty, Eldon R. Rene*, and Piet N. L. Lens |
Address |
UNESCO-IHE Institute for Water Education, 2601 DA Delft, The Netherlands |
Bibliography |
Journal of Microbiology, 57(9),738–747, 2019,
|
DOI |
10.1007/s12275-019-9042-6
|
Key Words |
Phanerochaete chrysosporium, Delftia lacustris,
phenol, selenite, Se(0) nanoparticles |
Abstract |
The simultaneous removal of phenol and selenite from synthetic
wastewater was investigated by adopting two different
co-culturing techniques using the fungus Phanerochaete chrysosporium
and the bacterium Delftia lacustris. Separately grown
biomass of the fungus and the bacterium (suspended co-culture)
was incubated with different concentrations of phenol
(0–1,200 mg/L) and selenite (10 mg/L). The selenite ions were
biologically reduced to extracellular Se(0) nanoparticles (3.58
nm diameter) with the simultaneous degradation of up to 800
mg/L of phenol. Upon growing the fungus and the bacterium
together using an attached growth co-culture, the bacterium
grew as a biofilm onto the fungus. The extracellularly produced
Se(0) in the attached growth co-culture had a minimum
diameter of 58.5 nm. This co-culture was able to degrade
completely 50 mg/L phenol, but was completely inhibited
at a phenol concentration of 200 mg/L. |