| Address |
1R&D Division, VITABIO, Inc., Daejeon 34025, Republic of Korea, 2Biological Resources Utilization Department, National Institute of Biological Resources, Incheon 22689, Republic of Korea, 3Microorganism Resources Division, National Institute of Biological Resources, Incheon 22689, Republic of Korea, 4Department of Biology, Jeju National University, Jeju 63243, Republic of Korea |
| Abstract |
A Gram-stain-positive, oxidase- and catalase-positive, motile,
aerobic, and rod-shaped bacterial strain, designated as
DCT-5T, was isolated from a native plant belonging to the genus
Campanula at Dokdo island, Republic of Korea. Growth
of the strain DCT-5T was observed at 15–37°C (optimum
30°C) on R2A broth, pH 6.0–8.0 (optimum 7.0), and 0–5%
(w/v) NaCl concentration (optimum 0%). The 16S rRNA gene
sequence analysis revealed that strain DCT-5T was most closely
related to Arthrobacter silviterrae KIS14-16T, Arthrobacter
livingstonensis LI2T, Arthrobacter stackebrandtii CCM
2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus
CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with
pairwise sequence similarities of 98.76%, 97.47%, 97.25%,
97.11%, 97.11%, and 97.00%, respectively. The DNA G+C
content of strain DCT-5T was 64.7 mol%, and its DNA–DNA
relatedness values with A. silviterrae KIS14-16T, A. livingstonensis
LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus
GP3T, A. ramosus CCM 1646T, and A. cryoconiti
Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%,
34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.
The major quinone was MK-9(H2) and major fatty acids were
anteiso-C15:0, anteiso-C17:0, iso-C15:0, and iso-C16:0. The polar
lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol
(PG), phosphatidylinositol (PI), unidentified glycolipid
(GL), two unidentified aminophospholipids (APLs), and three
unidentified lipids (Ls). The peptidoglycan type was A3α.
On the basis of phenotypic, phylogenetic, genotypic, and chemotaxonomic
characteristics, strain DCT-5T represents a
novel species of the genus Arthrobacter, for which the name
Arthrobacter dokdonellae sp. nov. is proposed. The type strain
is DCT-5T (= KCTC 49189T = LMG 31284T). |
