Title |
Molecular genomic characterization of severe fever with thrombocytopenia syndrome virus isolates from South Korea |
Author |
Yu Jung Won1, Lae Hyung Kang1, Sung Geun Lee2, Seung Won Park3, Jae Ik Han4, and Soon Young Paik1* |
Address |
1Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea, 2Korea Zoonosis Research Institute, Chonbuk National University, Iksan 54596, Republic of Korea, 3Department of Biotechnology, Daegu Catholic University, Gyeongsan 38430, Republic of Korea, 4Laboratory of Wildlife Diseases, College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Republic of Korea |
Bibliography |
Journal of Microbiology, 57(10),927–937, 2019,
|
DOI |
10.1007/s12275-019-9174-8
|
Key Words |
SFTS, SFTSV, whole-genome sequencing, Phlebovirus,
South Korea, phylogeny |
Abstract |
Severe fever with thrombocytopenia syndrome (SFTS) is a
tick-borne emerging infectious disease caused by the SFTS
virus (SFTSV) and is a threat to public health due to its high
fatality rate. However, details on tick-to-human transmission
of SFTSV are limited. In this study, we determined the wholegenome
sequence of a South Korean SFTSV strain (CUKJJ01),
compared it to those of other recent human SFTSV
isolates, and identified the genetic variations and relationships
among the SFTSV strains. The genome of CUK-JJ01
was consistent with the genome of other members of the genus
Phlebovirus, including the large (L), medium (M), and
small (S) segments of 6368, 3378, and 1744 nucleotides, respectively.
Based on amino acid sequences of the M and S
segments, which are used to distinguish the six SFTSV genotypes,
CUK-JJ01 was classified as genotype B. Segment analysis
revealed that the L, M, and S segments were 97.49%,
97.18%, and 97.94% similar to those of KAJNH2/2013/
Korea, ZJZHSH-FDE/2012/China, and KADGH/2013/Korea,
respectively. Currently, only few studies on SFTSV have been
conducted in Korean population and most were limited to
serological analysis. Although the present study has limitations
in terms of number of sample analyzed, the findings
may serve as basis to understand the transmission and spread
of SFTSV, as well as for the development of diagnostic and
detection methods for viral recombinants by comparing
the whole genome sequence of SFTSV isolates from South
Korea and that of foreign isolates. |