Title |
Methylobacterium terrae sp. nov., a radiation-resistant bacterium isolated from gamma ray-irradiated soil |
Author |
Jiyoun Kim1, Geeta Chhetri1, Inhyup Kim1, Hyungdong Kim1, Myung Kyum Kim2, and Taegun Seo*1 |
Address |
1Department of Life Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea, 2Department of Bio & Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul 01797, Republic of Korea |
Bibliography |
Journal of Microbiology, 57(11),959–966, 2019,
|
DOI |
10.1007/s12275-019-9007-9
|
Key Words |
Methylobacterium, new taxon, gamma ray-resistance,
phylogenetic analysis, cell morphology |
Abstract |
A Gram-stain-negative, asporogenous, aerobic rods, motile by
means of a single polar flagellum, catalase- and oxidase-positive,
methylotrophic bacterium, designated 17Sr1-28T, was
isolated from gamma ray-irradiated soil. The 16S rRNA gene
sequence analysis showed that strain 17Sr1-28T was phylogenetically
related to Methylobacterium currus PR1016AT (96.8%),
Methylobacterium platani PMB02T (96.2%), Methylobacterium
aquaticum DSM 16371T (96.3%), Methylobacterium tarhaniae
N4211T (96.4%), Methylobacterium frigidaeris IER25-16T
(95.8%), and Methylobacterium organophilum JCM 2833T
(92.7%). The G+C content calculated based on genome sequence
was 71.6%. The average nucleotide identity and in
silico DNA-DNA hybridization values between strain 17Sr1-
28T and M. currus, M. platani, M. aquaticum, M. tarhaniae,
M. frigidaeris, and M. organophilum were 77.7–90.4% and
22–39.6%, respectively. The major fatty acids of strain 17Sr1-
28T were summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), and
summed feature 3 (C16:1 ω7c and/or C16:1 ω6c). The predominant
quinone was ubiquinone 10 and the major polar lipids
were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine,
and phosphatidylglycerol. On the basis
of the data from phenotypic tests and genotypic differences
between strain 17Sr1-28T and its close phylogenetic relatives,
strain 17Sr1-28T represents a new species belonging to the
genus Methylobacterium, for which the name Methylobacterium
terrae sp. nov. (= KCTC 52904T = NBRC 112873T) is
proposed. |