| Title | Cohnella abietis sp. nov., isolated from Korean fir (Abies koreana) rhizospheric soil of Halla mountain |
|---|---|
| Author | Lingmin Jiang1,2, Sophea Pheng1,3, Keun Chul Lee1, Se Won Kang1, Jae Cheol Jeong1, Cha Young Kim1, Hyeong Cheol Park4, Dae-Hyuk Kim2, Suk Weon Kim1, Song-Gun Kim*1,3, and Jiyoung Lee*1 |
| Address | 1Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea, 2Department of Bioactive Materials, Chonbuk National University, Jeonju 54896, Republic of Korea, 3University of Science and Technology, Daejeon 34113, Republic of Korea, 4Division of Ecological Conservation, Bureau of Ecological Research, Seocheon 33657, Republic of Korea |
| Bibliography | Journal of Microbiology, 57(11),953–958, 2019, |
| DOI | 10.1007/s12275-019-9136-1 |
| Key Words | Cohnella abietis, taxonomy, genome, Korea fir |
| Abstract | A strictly aerobic, motile, endospore-forming, rod-shaped bacterium, designated HS21T, was isolated from rhizospheric soil of the Korean fir tree (Abies koreana) from Halla mountain on Jeju island, Korea. Growth of strain HS21T was observed at pH 6.0–8.0 (optimum: pH 7.0), 0–2% (w/v) NaCl and 4–30°C (optimum: 25°C). A comparative analysis of 16S rRNA gene sequences showed that strain HS21T was most closely related to Cohnella luojiensis HY-22RT (97.6%), followed by C. lupini RLAHU4BT (97.4%) and C. collisoli NKM- 5T (97.2%). The genome of strain HS21T comprised a circular chromosome of 7,059,027 bp with 44.8% G + C content. The DNA-DNA relatedness values between strain HS21T and C. luojiensis HY-22RT and C. lupini RLAHU4BT were 18.1% and 13.8%, respectively. The major cellular fatty acids (> 5%) of the isolate were anteiso-C15:0, iso-C16:0, C16:0, and iso-C15:0. The polar lipids present were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, lysylphosphatidylglycerol, and three unidentified aminophospholipids. Based on its phenotypic, phylogenetic, genomic, and chemotaxonomic properties, strain HS21T represents a novel species of the genus Cohnella, for which the name Cohnella abietis sp. nov. is proposed. The type strain is HS21T (= KCTC 43028T = CCTCC AB 2019010T). |