Title |
Methyltransferase of a cell culture-adapted hepatitis E inhibits the MDA5 receptor signaling pathway |
Author |
Jinjong Myoung*, Jeong Yoon Lee, and Kang Sang Min |
Address |
Korea Zoonosis Research Institute, Genetic Engineering Research Institute and Department of Bioactive Material Science, Jeonbuk National University, Jeonju 54896, Republic of Korea |
Bibliography |
Journal of Microbiology, 57(12),1126-1131, 2019,
|
DOI |
10.1007/s12275-019-9478-8
|
Key Words |
hepatitis E virus, methyltransferase, interferon |
Abstract |
Hepatitis E virus (HEV) is a causative agent of acute hepatitis
and jaundice. The number of human infections is approximated
to be over 20 million cases per year. The transmission
is mainly via the fecal-oral route and contaminated water and
food are considered to be a major source of infection. As a
mouse model is not available, a recent development of a cell
culture-adapted HEV strain (47832c) is considered as a very
important tools for molecular analysis of HEV pathogenesis
in cells. Previously, we demonstrated that HEV-encoded methyltransferase
(MeT) encoded by the 47832c strain inhibits
MDA5- and RIG-I-mediated activation of interferon β (IFN-β)
promoter. Here, we report that MeT impairs the phosphorylation
and activation of interferon regulatory factor 3 and the
p65 subunit of NF-κB in a dose-dependent manner. In addition,
the MeT encoded by the 47832c, but not that of HEV
clinical or field isolates (SAR-55, Mex-14, KC-1, and ZJ-1),
displays the inhibitory effect. A deeper understanding of MeTmediated
suppression of IFN-β expression would provide
basis of the cell culture adaptation of HEV. |