| Title | H2 Metabolism revealed by metagenomic analysis of subglacial sediment from East Antarctica |
|---|---|
| Author | Zhifeng Yang1,2,3, Yu Zhang2,3, Yongxin Lv2,3, Wenkai Yan2,3, Xiang Xiao2,3, Bo Sun1, and Hongmei Ma1* |
| Address | 1SOA Key Laboratory for Polar Science, Polar Research Institute of China, P. R. China, 2State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, P. R. China, 3School of Oceanography, Shanghai Jiao Tong University, P. R. China |
| Bibliography | Journal of Microbiology, 57(12),1095-1104, 2019, |
| DOI | 10.1007/s12275-019-9366-2 |
| Key Words | subglacial, Antarctic, hydrogen, chemoautotrophy, nitrate, metagenome |
| Abstract | Subglacial ecosystems harbor diverse chemoautotrophic microbial communities in areas with limited organic carbon, and lithological H2 produced during glacial erosion has been considered an important energy source in these ecosystems. To verify the H2-utilizing potential there and to identify the related energy-converting metabolic mechanisms of these communities, we performed metagenomic analysis on subglacial sediment samples from East Antarctica with and without H2 supplementation. Genes coding for several [NiFe]- hydrogenases were identified in raw sediment and were enriched after H2 incubation. All genes in the dissimilatory nitrate reduction and denitrification pathways were detected in the subglacial community, and the genes coding for these pathways became enriched after H2 was supplied. Similarly, genes transcribing key enzymes in the Calvin cycle were detected in raw sediment and were also enriched. Moreover, key genes involved in H2 oxidization, nitrate reduction, oxidative phosphorylation, and the Calvin cycle were identified within one metagenome-assembled genome belonging to a Polaromonas sp. As suggested by our results, the microbial community in the subglacial environment we investigated consisted of chemoautotrophic populations supported by H2 oxidation. These results further confirm the importance of H2 in the cryosphere. |