| Abstract |
A yellow pigmented, Gram-stain-negative, strictly aerobic,
rod-shaped, motile by means of gliding, catalase and oxidase
positive bacterium, designated strain DS2-AT, was isolated
from soil. Growth was observed at 4–32°C (optimum, 28°C),
pH 6–9 (optimum, 7.0), and with 0–0.25% (w/v) NaCl (optimum,
0%). Phylogenetic analysis of 16S rRNA gene sequence
revealed that strain DS2-AT belonged to the genus Flavobacterium
and was most closely related to Flavobacterium
aquatile LMG 4008T (96.4%), Flavobacterium terrae DSM
18829T (95.6%), Flavobacterium vireti THG-SM1T (95.5%),
Flavobacterium inkyongense IMCC27201T (95.4%), Flavobacterium
brevivitae TTM-43T (95.2%), and Flavobacterium
cucumis DSM 18830T (95.2%). Strain DS2-AT produces flexirubin-
type pigments. The major fatty acids were iso-C15:0,
iso-C17:0 3-OH, and iso-C15:0 3-OH. The major respiratory
quinone was identified as menaquinone-6. The major polar
lipid was found to be phosphatidylethanolamine. The average
nucleotide identity values between strain DS2-AT and selected
taxa, F. aquatile LMG 4008T, F. terrae DSM 18829T,
and F. cucumis DSM 18830T, were 72, 72.7, and 71.6%, respectively.
The draft genome of strain DS2-AT has a number
of 14 contigs, scaffold N50 of 476,310 bp and a total size of
3,563,867 bp. Additionally, strain DS2-AT contains 3,127 of
gene, 41 of tRNA, 6 of rRNA, and 3 of ncRNA. The DNA G
+ C content of stain DS2-AT was 40.7 mol%. Based on phylogenetic
and phenotypic analyses, strain DS2-AT is considered
as a novel species of the genus Flavobacterium, for which
the name Flavobacterium humi sp. nov., (type strain DS2-AT
= KACC 19715T = JCM 32786T) has been proposed. |
