| Title | Anaerotignum faecicola sp. nov., isolated from human faeces |
|---|---|
| Author | Seung-Hyeon Choi1+, Ji-Sun Kim1+, Jam-Eon Park1, Keun Chul Lee1, Mi Kyung Eom1, Byeong Seob Oh1, Seung Yeob Yu1, Se Won Kang1, Kook-Il Han1, Min Kuk Suh1, Dong Ho Lee2, Hyuk Yoon2, Byung-Yong Kim3, Je Hee Lee3, Ju Huck Lee1, Jung-Sook Lee1,4*, and Seung-Hwan Park1* |
| Address | 1Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup 56212, Republic of Korea, 2Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea, 3ChunLab, Inc., Seoul 06725, Republic of Korea, 4University of Science and Technology (UST), Daejeon 34113, Republic of Korea |
| Bibliography | Journal of Microbiology, 57(12),1073-1078, 2019, |
| DOI | 10.1007/s12275-019-9268-3 |
| Key Words | Anaerotignum faecicola, human faeces, taxonomy, microbiota |
| Abstract | A strictly anaerobic bacterium, designated as strain KGMB- 03357T, was isolated from the faeces of a healthy Korean selected by Bundang Seoul National University based on health status. Cells of strain KGMB03357T are Gram-stain-positive, non-motile, non-spore-forming, and observed as straight or curved rods. The isolate grew at 10–45°C (optimum temperature of 40°C) and a pH range of 5.1–10.5 (optimum pH of 6.8). Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%). The ANI values between strain KGMB 03357T and members of the genus Anaerotignum were 73.3–71.0%, which are below the ANI criterion for interspecies identity. The DNA G + C content based on the whole-genome sequence is 47.3 mol%. The major cellular fatty acids of strain KGMB03357T are C16:0, C18:0, C18:1 cis 9, and anteiso-C15:0. Strain KGMB03357T contains meso-diaminopimelic acid as the diagnostic amino acid in the cell wall peptidoglycan. Based on the phenotypic, phylogenetic, and genomic properties, strain KGMB 03357T represents a novel species of the genus Anaerotignum, for which the name Anaerotignum faecicola sp. nov. is proposed. The type strain is KGMB03357T (= KCTC 15736T = DSM 107953T). |