| Title | Identification and characterization of a novel light-induced promoter for recombinant protein production in Pleurotus ostreatus |
|---|---|
| Author | Chaomin Yin1,2*, Xiuzhi Fan1, Kun Ma1, Zheya Chen1, Defang Shi1, Fen Yao1, Hong Gao1,2, and Aimin Ma3* |
| Address | 1Institute of Agro-Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, P. R. China, 2National Research and Development Center for Edible Fungi Processing (Wuhan), Wuhan 430064, P. R. China, 3College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, P. R. China |
| Bibliography | Journal of Microbiology, 58(1),39-45, 2020, |
| DOI | 10.1007/s12275-020-9230-4 |
| Key Words | Pleurotus ostreatus, lectin, promoter, Agrobacterium tumefaciens, light-induction |
| Abstract | A lectin gene (plectin) with a high level of expression was previously identified by comparative transcriptome analysis of Pleurotus ostreatus. In this study, we cloned a 733-bp DNA fragment from the start codon of the plectin gene. Sequence analysis showed that the plectin promoter (Plp) region contained several eukaryotic transcription factor binding motifs, such as the TATA-box, four possible CAAT-box, light responsiveness motifs and MeJA-responsiveness motifs. To determine whether the Plp promoter was a light-regulated promoter, we constructed an expression vector with the fused egfp-hph fragment under the control of the Plp promoter and transformed P. ostreatus mycelia via Agrobacterium tumefaciens. PCR and Southern blot analyses confirmed the Plpegfp- hph fragment was integrated into the chromosomal DNA of transformants. qRT-PCR, egfp visualization, and intracellular egfp determination experiments showed the Plp promoter could be a light-induced promoter that may be suitable for P. ostreatus genetic engineering. This study lays the foundation for gene homologous expression in P. ostreatus. |