Title |
Sterilization efficiency of pathogen-contaminated cottons in a laundry machine |
Author |
Yoonjae Shin1, Jungha Park2, and Woojun Park1* |
Address |
1Laboratory of Molecular Environmental Microbiology, Department of Environmental Science and Ecological Engineering, Korea University, Seoul 02841, Republic of Korea, 2Digital Appliances Business, Samsung Electronics, Suwon 16677, Republic of Korea |
Bibliography |
Journal of Microbiology, 58(1),30–38, 2020,
|
DOI |
10.1007/s12275-020-9391-1
|
Key Words |
Acinetobacter baumannii, desiccation tolerance,
dry conditions, drying machine, Staphylococcus aureus, washing
machine |
Abstract |
Pathogenic bacteria on abiotic surfaces such as fabrics, bedding,
patient wears, and surgical tools are known to increase
the risk of bacterial diseases in infants and the elderly. The
desiccation tolerance of bacteria affects their viability in cotton.
Thus, washing and drying machines are required to use
conditions that ensure the sterilization of bacteria in cotton.
The objective of this study is to determine the effects of various
sterilization conditions of washing and drying machines
on the survival of three pathogenic bacteria (Acinetobacter
baumannii, Pseudomonas aeruginosa, and Staphylococcus
aureus) commonly presented in contaminated cotton and two
non-pathogenic bacteria (Bacillus subtilis and Escherichia coli)
in cotton. High survival rates of A. baumannii and S. aureus
in desiccated cotton were observed based on scanning electron
microscope and replicate organism direct agar contact
assay. The survival rates of A. baumannii and S. aureus exposed
in desiccated cotton for 8 h were higher (14.4 and 5.0%,
respectively) than those of other bacteria (< 0.5%). All tested
bacteria were eradicated at low-temperature (< 40°C) washing
with activated oxygen bleach (AOB). However, bacterial
viability was shown in low temperature washing without AOB.
High-temperature (> 60°C) washing was required to achieve
99.9% of the sterilization rate in washing without AOB. The
sterilization rate was 93.2% using a drying machine at 60°C
for 4 h. This level of sterilization was insufficient in terms
of time and energy efficiency. High sterilization efficiency
(> 99.9%) at 75°C for 3 h using a drying machine was confirmed.
This study suggests standard conditions of drying
machines to remove bacterial contamination in cotton by
providing practical data. |