Title |
Paraflavitalea soli gen. nov., sp. nov., isolated from greenhouse soil |
Author |
Jun Heo, Hang-Yeon Weon, Hayoung Cho, Seung-Beom Hong, Jeong-Seon Kim, Soo-Jin Kim*, and Soon-Wo Kwon |
Address |
Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Jeollabuk-do 55365, Republic of Korea |
Bibliography |
Journal of Microbiology, 58(1),17-23, 2020,
|
DOI |
10.1007/s12275-020-9236-y
|
Key Words |
new genus, new species, Paraflavitalea soli, Chitinophagaceae |
Abstract |
A bacterial strain designated 5GH32-13T was isolated from
greenhouse soil in Yongin-city, Republic of Korea. Cells were
Gram-stain-negative, strictly aerobic, motile rods of two different
shapes. The strain was catalase-positive and oxidasenegative.
Flexirubin-like pigments were not detected. β-Carotene
was produced. The strain grew in the range of 10–37°C
(optimum of 28–30°C) and pH 6–8 (optimum of pH 7) and
tolerated up to 1% (w/v) NaCl (optimum of 0%). According
to the 16S rRNA gene sequence comparison, strain 5GH32-
13T shared a sequence similarity of less than 96.0% with all
validly named taxa, having the highest sequence similarity
with Pseudoflavitalea soli KIS20-3T (95.8%), Pseudoflavitalea
rhizosphaerae T16R-265T (95.4%), Flavitalea gansuensis
JCN-23T (95.3%), Pseudobacter ginsenosidimutans Gsoil 221T
(95.3%), and Flavitalea populi HY-50RT (95.2%). A phylogenetic
tree showed that strain 5GH32-13T was not grouped
consistently into any specific genus. Its only polyamine was
homospermidine, and its major fatty acids (> 10% of total
fatty acids) were iso-C15:0, iso-C17:0 3-OH, and iso-C15:1 G. The
strain’s only respiratory quinone was MK-7, and its polar
lipids were phosphatidylethanolamine, one unidentified phospholipid,
six unidentified aminolipids and four unidentified
lipids. Its DNA G + C content was 47.5 mol%. The results
from chemotaxonomic, phenotypic and phylogenetic analyses
indicated that strain 5GH32-13T represents a novel species
of a novel genus of the family Chitinophagaceae, and the
name Paraflavitalea soli gen. nov., sp. nov. is proposed. The
type strain is 5GH32-13T (= KACC 17331T = JCM 33061T). |