Title |
[Protocol]Rapid method for chromatin immunoprecipitation (ChIP) assay in a dimorphic fungus, Candida albicans |
Author |
Jueun Kim1,2 and Jung-Shin Lee1,2* |
Address |
1Department of Molecular Bioscience, 2Critical Zone Frontier Research Laboratory, Kangwon National University, Chuncheon 24341, Republic of Korea |
Bibliography |
Journal of Microbiology, 58(1),11–16, 2020,
|
DOI |
10.1007/s12275-020-9143-2
|
Key Words |
Candida albicans, chromatin immunoprecipitation
(ChIP) assay, dimorphic fungus |
Abstract |
A chromatin immunoprecipitation (ChIP) assay is a method
to identify how much a protein of interest binds to the DNA
region. This method is indispensable to study the mechanisms
of how the transcription factors or chromatin modifications
regulate the gene expression. Candida albicans is a dimorphic
pathogenic fungus, which can change its morphology very rapidly
from yeast to hypha in response to the environmental
signal. The morphological change of C. albicans is one of the
critical factors for its virulence. Therefore, it is necessary to
understand how to regulate the expression of genes for C.
albicans to change its morphology. One of the essential methods
for us to understand this regulation is a ChIP assay.
There have been many efforts to optimize the protocol to lower
the background signal and to analyze the results accurately
because a ChIP assay can provide very different results even
with slight differences in the experimental procedure. We
have optimized the rapid and efficient ChIP protocol so that
it could be applied equally for both yeast and hyphal forms of
C. albicans. Our method in this protocol is also comparatively
rapid to the method widely used. In this protocol, we described
our rapid method for the ChIP assay in C. albicans in
detail. |