Title |
[Minireview]Recent advances in genetic engineering tools based on synthetic biology |
Author |
Jun Ren, Jingyu Lee, and Dokyun Na* |
Address |
School of Integrative Engineering, Chung-Ang University, Seoul 06974, Republic of Korea |
Bibliography |
Journal of Microbiology, 58(1),1–10, 2020,
|
DOI |
10.1007/s12275-020-9334-x
|
Key Words |
CRISPR, MAGE, promoter engineering, CRISPRi,
CRISPRa, synthetic sRNA, metabolic engineering, genomescale
engineering |
Abstract |
Genome-scale engineering is a crucial methodology to rationally
regulate microbiological system operations, leading
to expected biological behaviors or enhanced bioproduct yields.
Over the past decade, innovative genome modification
technologies have been developed for effectively regulating
and manipulating genes at the genome level. Here, we discuss
the current genome-scale engineering technologies used for
microbial engineering. Recently developed strategies, such
as clustered regularly interspaced short palindromic repeats
(CRISPR)-Cas9, multiplex automated genome engineering
(MAGE), promoter engineering, CRISPR-based regulations,
and synthetic small regulatory RNA (sRNA)-based knockdown,
are considered as powerful tools for genome-scale engineering
in microbiological systems. MAGE, which modifies
specific nucleotides of the genome sequence, is utilized as a
genome-editing tool. Contrastingly, synthetic sRNA, CRISPRi,
and CRISPRa are mainly used to regulate gene expression
without modifying the genome sequence. This review introduces
the recent genome-scale editing and regulating technologies
and their applications in metabolic engineering. |