Title |
Parahaliea maris sp. nov., isolated from surface seawater and emended description of the genus Parahaliea |
Author |
Yang Liu1, Juan Du1, Jun Zhang1, Qiliang Lai2, Zongze Shao2*, and Honghui Zhu1* |
Address |
1State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, P. R. China, 2Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, State Key Laboratory Breeding Base of Marine Genetic Resources, Fujian Key Laboratory of Marine Genetic Resources, Xiamen 361005, P. R. China |
Bibliography |
Journal of Microbiology, 58(2),92-98, 2020,
|
DOI |
10.1007/s12275-020-9405-z
|
Key Words |
Parahaliea maris, polyphasic taxonomy, genomic
analysis, novel species |
Abstract |
A Gram-stain-negative, strictly aerobic, short-rod-shaped,
and non-motile bacterial strain designated HSLHS9T was
isolated from surface seawater collected from the South China
Sea. Strain HSLHS9T could grow at 15–41°C (optimum 28°C),
at pH 5.0–9.0 (optimum 6.0–7.0), and in 0–7% (w/v) NaCl
(optimum 2–3%). Phylogenetic analysis based on 16S rRNA
gene sequences indicated that strain HSLHS9T shared high
identities with the closely related Parahaliea aestuarii S2-26T
(98.6%) and Parahaliea mediterranea 7SM29T (97.8%) and
formed a distinct lineage within the genus Parahaliea. Wholegenome
sequencing of strain HSLHS9T revealed the size of 4.8
Mbp and DNA G + C content of 61.8 mol%. Strain HSLHS9T
shared the digital DNA-DNA hybridization values of 22.4%
and 23.0%, and the average nucleotide identities of 79.7%
and 79.9%, respectively, with the two type strains above. The
predominant cellular fatty acids of the strain were summed
feature 8 (C18:1 ω6c and/or C18:1 ω7c), summed feature 3 (C16:1
ω7c and/or C16:1 ω6c), C17:1 ω8c, and C16:0. The sole isoprenoid
quinone was identified as Q-8. The polar lipids were phosphatidylglycerol,
diphosphatidylglycerol, phosphatidylethanolamine,
aminolipid, and two glycolipids. Based on taxonomic
data obtained in this study, it is suggested that strain
HSLHS9T represents a novel species of the genus Parahaliea,
for which the name Parahaliea maris sp. nov. is proposed.
The type strain is HSLHS9T (= MCCC 1A06717T = KCTC
52307T). An emended description of the genus Parahaliea
is also provided. |