Title |
Lactobacillus crispatus and its enolase and glutamine synthetase influence interactions between Neisseria gonorrhoeae and human epithelial cells |
Author |
Jagoda Płaczkiewicz1, Paulina Chmiel1, Ewelina Malinowska1, Pawel Bącal2, and Agnieszka Kwiatek1* |
Address |
1Department of Virology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland, 2Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Ks. Trojdena 4, 02-109 Warsaw, Poland |
Bibliography |
Journal of Microbiology, 58(5),405-414, 2020,
|
DOI |
10.1007/s12275-020-9505-9
|
Key Words |
Neisseria gonorrhoeae, enolase, glutamine synthetase,
microbiota, Lactobacillus crispatus |
Abstract |
Neisseria gonorrhoeae, an obligatory human pathogen causes
the sexually transmitted disease gonorrhea, which remains
a global health problem. N. gonorrhoeae primarily infects the
mucosa of the genitourinary tract, which in women, is colonized
by natural microbiota, dominated by Lactobacillus spp.,
that protect human cells against pathogens. In this study, we
demonstrated that precolonization of human epithelial cells
with Lactobacillus crispatus, one of the most prevalent bacteria
in the female urogenital tract, or preincubation with the
L. crispatus enolase or glutamine synthetase impairs the adhesion
and invasiveness of N. gonorrhoeae toward epithelial
cells, two crucial steps in gonococcal pathogenesis. Furthermore,
decreased expression of genes encoding the proinflammatory
cytokines, TNFα and CCL20, which are secreted as
a consequence of N. gonorrhoeae infection, was observed in
N. gonorrhoeae-infected epithelial cells that had been precolonized
with L. crispatus or preincubated with enolase and
glutamine synthetase. Thus, our results indicate that the protection
of human cells against N. gonorrhoeae infection is a
complex process and that L. crispatus and its proteins enolase
and glutamine synthetase can have a potential role in protecting
epithelial cells against gonococcal infection. Therefore,
these results are important since disturbances of the microbiota
or of its proteins can result in dysbiosis, which is associated
with increased susceptibility of epithelium to pathogens. |