Title |
Endophytic bacterial and fungal microbiota in different cultivars of cassava (Manihot esculenta Crantz) |
Author |
Hong Li1†, Chengliang Yan2†, Yanqiong Tang1, Xiang Ma1, Yinhua Chen2, Songbi Chen3, Min Lin4, and Zhu Liu1* |
Address |
1Key Laboratory of Tropical Biological Resources of Ministry of Education, School of Life and Pharmaceutical Sciences, Hainan University, Haikou, 570228, P. R. China, 2School of Tropical Crops, Hainan University, Haikou, 570228, P. R. China, 3Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, P. R. China, 4Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China |
Bibliography |
Journal of Microbiology, 58(7),614–623, 2020,
|
DOI |
10.1007/s12275-020-9565-x
|
Key Words |
endophytic bacteria, endophytic fungi, cassava,
susceptibility, high-throughput sequencing |
Abstract |
Endophytes colonize tissues of healthy host plants and play
a crucial role in plant growth and development. However,
little attention has been paid to the endophytes of tuber crops
such as cassava, which is used as a staple food by approximately
800 million people worldwide. This study aimed to
elucidate the diversity and composition of endophytic bacterial
and fungal communities in different cassava cultivars
using high-throughput sequencing. Although no significant
differences in richness or diversity were observed among the
different cassava cultivars, the community compositions were
diverse. Two cultivars (SC124 and SC205) tolerant to root rot
exhibited similar community compositions, while two other
cultivars (SC10 and SC5), which are moderately and highly
susceptible to root rot, respectively, harboured similar community
compositions. Proteobacteria, Firmicutes, and Ascomycota
dominated the endophyte assemblages, with Weissella,
Serratia, Lasiodiplodia, Fusarium, and Diaporthe being the
predominant genera. The differentially abundant taxonomic
clades between the tolerant and susceptible cultivars were
mainly rare taxa, such as Lachnoclostridium_5, Rhizobium,
Lampropedia, and Stenotrophomonas. These seemed to be key
genera that affected the susceptibility of cassava to root rot.
Moreover, the comparison of KEGG functional profiles revealed
that ‘Environmental adaptation’ category was significantly
enriched in the tolerant cultivars, while ‘Infectious
diseases: Parasitic’ category was significantly enriched in the
susceptible cultivars. The present findings open opportunities
for further studies on the roles of endophytes in the susceptibility
of plants to diseases. |