Title |
Melatonin attenuates microbiota dysbiosis of jejunum in short-term sleep deprived mice |
Author |
Ting Gao, Zixu Wang, Jing Cao, Yulan Dong, and Yaoxing Chen* |
Address |
College of Veterinary Medicine, China Agricultural University, Beijing 100193, P. R. China |
Bibliography |
Journal of Microbiology, 58(7),588–597, 2020,
|
DOI |
10.1007/s12275-020-0094-4
|
Key Words |
sleep deprivation, melatonin, jejunum, microbiota,
oxidative stress |
Abstract |
Our study demonstrated that sleep deprivation resulted in
homeostasis disorder of colon. Our study goes deeper into the
positive effects of melatonin on small intestinal microbiota
disorder caused by sleep deprivation. We successfully established
a multiplatform 72 h sleep deprivation mouse model
with or without melatonin supplementation, and analyzed the
change of small intestinal microbiota using high-throughput
sequencing of the 16S rRNA. We found melatonin supplementation
suppressed the decrease of plasma melatonin level
in sleep deprivation mice. Meanwhile, melatonin supplementation
improved significantly the reduction in OTU numbers
and the diversity and richness of jejunal microbiota and
the abundance of Bacteroidaeae and Prevotellaceae, as well
as an increase in the Firmicutes-to-Bacteroidetes ratio and
the content of Moraxellaceae and Aeromonadaceae in the jejunum
of sleep deprived-mice. Moreover, melatonin supplementation
reversed the change of metabolic pathway in sleep
deprived-mice, including metabolism, signal transduction
mechanisms and transcription etc, which were related to intestinal
health. Furthermore, melatonin supplementation inverted
the sleep deprivation-induced a decline of anti-inflammatory
cytokines (IL-22) and an increase of the ROS and proinflammatory
cytokines (IL-17) in jejunum. These findings
suggested that melatonin, similar to a probiotics agent, can
reverse sleep deprivation-induced small intestinal microbiota
disorder by suppressing oxidative stress and inflammation
response. |