Title |
Burkholderia thailandensis outer membrane vesicles exert antimicrobial activity against drug-resistant and competitor microbial species |
Author |
Yihui Wang1, Joseph P. Hoffmann1, Chau-Wen Chou2, Kerstin Höner zu Bentrup1, Joseph A. Fuselier3, Jacob P. Bitoun1, William C. Wimley4, and Lisa A. Morici1 |
Address |
1Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA, USA, 2Department of Chemistry, University of Georgia Franklin College of Arts and Sciences, Athens, GA, USA, 3Department of Medicine, Tulane University School of Medicine, New Orleans, LA, USA, 4Department of Biochemistry and Molecular Biology, Tulane University School of Medicine, New Orleans, LA, USA |
Bibliography |
Journal of Microbiology, 58(7),550–562, 2020,
|
DOI |
10.1007/s12275-020-0028-1
|
Key Words |
antimicrobial resistance, outer membrane vesicles,
biofilm, bacteria, fungi |
Abstract |
Gram-negative bacteria secrete outer membrane vesicles
(OMVs) that play critical roles in intraspecies, interspecies,
and bacteria-environment interactions. Some OMVs, such
as those produced by Pseudomonas aeruginosa, have previously
been shown to possess antimicrobial activity against
competitor species. In the current study, we demonstrate that
OMVs from Burkholderia thailandensis inhibit the growth
of drug-sensitive and drug-resistant bacteria and fungi. We
show that a number of antimicrobial compounds, including
peptidoglycan hydrolases, 4-hydroxy-3-methyl-2-(2-nonenyl)-
quinoline (HMNQ) and long-chain rhamnolipid are
present in or tightly associate with B. thailandensis OMVs.
Furthermore, we demonstrate that HMNQ and rhamnolipid
possess antimicrobial and antibiofilm properties against methicillin-
resistant Staphylococcus aureus (MRSA). These findings
indicate that B. thailandensis secretes antimicrobial OMVs
that may impart a survival advantage by eliminating competition.
In addition, bacterial OMVs may represent an untapped
resource of novel therapeutics effective against biofilm-
forming and multidrug-resistant organisms. |