| Abstract |
A grey pink colored bacterium, strain t3-1-3T, was isolated
from the air at the foot of the Xiangshan Mountain in Beijing,
China. The cells are aerobic, Gram-stain-negative, non-sporeforming,
motile and coccoid-rod shaped (0.9–1.2 × 1.9–2.1
μm). Strain t3-1-3T was catalase-positive and oxidase-negative
and this strain grew at 4–42°C (optimum 28°C), a pH
of 4.0–9.0 (optimum pH 7.0) and under 0–2% (w/v) NaCl
(optimum 0–1% NaCl). A phylogenetic analysis based on 16S
rRNA gene sequences revealed that strain t3-1-3T was closely
related to Azohydromonas riparia UCM-11T (97.4% similarity),
followed by Azohydromonas australica G1-2T (96.8%)
and Azohydromonas ureilytica UCM-80T (96.7%). The genome
of strain t3-1-3T contains 6,895 predicted protein-encoding
genes, 8 rRNA genes, 62 tRNA genes and one sRNA
gene, as well as five potential biosynthetic gene clusters, including
clusters of genes coding for non-ribosomal peptide
synthetase (NRPS), bacteriocin and arylpolyene and two clusters
of genes for terpene. The predominant cellular fatty acids
(> 10.0% of the total) in strain t3-1-3T were summed feature
3 (C16:1ω7c and/or C16:1ω6c, 37.8%), summed feature 8
(C18:1ω7c and/or C18:1ω6c, 29.7%) and C16:0 (17.3%). Strain
t3-1-3T contained ubiquinone-8 (Q-8) as the predominant
respiratory quinone. The polar lipids of strain t3-1-3T comprised
phosphatidyl ethanolamine (PE), phosphatidyl glycerol
(PG), diphosphatidyl glycerol (DPG), an unidentified
glycolipid (GL), an unidentified aminophospholipid (APL),
two unidentified phospholipid (PL1-2) and five unidentified
lipid (L1-5). The DNA G + C content of the type strain
is 70.3%. The broader range of growth temperature, assimilation
of malic acid and trisodium citrate, presence of C18:3ω6c
and an unidentified glycolipid and absence of C12:0 2-OH and
C16:0iso differentiate strain t3-1-3T from related species. Based
on the taxonomic data presented in this study, we suggest
that strain t3-1-3T represents a novel species within the genus
Azohydromonas, for which the name Azohydromonas
aeria sp. nov. is proposed. The type strain of Azohydromonas
aeria is t3-1-3T (= CFCC 13393T = LMG 30135T). |
