| Title | Development of a real-time loop-mediated isothermal amplification method for the detection of severe fever with thrombocytopenia syndrome virus |
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| Author | Jae Woong Lee1, Yu-Jung Won1, Lae Hyung Kang1, Sung-Geun Lee2, Seung-Won Park3, and Soon-Young Paik1* |
| Address | 1Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea, 2Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 54596, Republic of Korea, 3Department of Biotechnology, Daegu Catholic University, Gyeongsan-si 38430, Republic of Korea |
| Bibliography | Journal of Microbiology, 58(8),711–715, 2020, |
| DOI | 10.1007/s12275-020-0109-1 |
| Key Words | severe fever with thrombocytopenia syndrome (STFS), reverse transcription loop-mediated isothermal amplification (LAMP), molecular diagnostics, virus detection |
| Abstract | Severe fever with thrombocytopenia syndrome (SFTS) is being reported annually in South Korea since its first detection there in 2010. The causal agent is a negative-strand RNA virus 80–100 nm in diameter. It causes fever, thrombocytopenia, leukocytopenia, gastrointestinal symptoms, and neural symptoms. The mortality rate of SFTS was 32.6% among 172 cases reported from 2012 to 2015 in South Korea. Thus, is necessary to develop an effective diagnostic method that selectively identifies the isolates circulating in South Korea. The real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay is a simple, rapid, and sensitive approach for molecular diagnosis. Here, we designed novel primers for this assay and found that the technique had very high specificity, sensitivity, and efficiency. This real-time RTLAMP approach using the novel primers developed herein can be applied for early diagnosis of SFTSV strains in South Korea to reduce the mortality rate of SFTS. |