Title |
Performance comparison of fecal preservative and stock solutions for gut microbiome storage at room temperature |
Author |
Chanhyeok Park1†, Kyeong Eui Yun1†, Jeong Min Chu1, Ji Yeon Lee1, Chang Pyo Hong1, Young Do Nam2, Jinuk Jeong3, Kyudong Han3*, and Yong Ju Ahn1* |
Address |
1Theragen Bio Co., Ltd., Suwon, Gyeonggi-do 16229, Republic of Korea, 2Research Group of Healthcare, Korea Food Research Institute, Jeollabuk-do 55365, Republic of Korea, 3Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 31116, Republic of Korea |
Bibliography |
Journal of Microbiology, 58(8),703–710, 2020,
|
DOI |
10.1007/s12275-020-0092-6
|
Key Words |
gut microbiome, stock solution, fecal collection
tube, metagenome, room temperature |
Abstract |
The gut microbiome, which is symbiotic within the human
body, assists in human digestion. It plays significant roles
in identifying intestinal disease as well as in maintaining a
healthy body with functional immune and metabolic activities.
To confirm the consistency of fecal intestinal microbial
research, it is necessary to study the changes in intestinal microbial
flora according to the fecal collection solution and
storage period. We collected fecal samples from three healthy
Korean adults. To examine the efficacy of fecal collection solution,
we used NBgene-Gut, OMNIgene-Gut, 70% ethanol
(Ethanol-70%), and RNAlater. The samples were stored for
up to two months at room temperature using three different
methods, and we observed changes in microbial communities
over time. We analyzed clusters of changes in the microbial
flora by observing fecal stock solutions and metagenome
sequencing performed over time. In particular, we confirmed
the profiling of alpha and beta diversity and microbial classification
according to the differences in intestinal environment
among individuals. We also confirmed that the microbial
profile remained stable for two months and that the microbial
profile did not change significantly over time. In addition,
our results suggest the possibility of verifying microbial
profiling even for long-term storage of a single sample. In conclusion,
collecting fecal samples using a stock solution rather
than freezing feces seems to be relatively reproducible and
stable for GUT metagenome analysis. Therefore, stock solution
tubes in intestinal microbial research can be used without
problems. |