Title |
Production and characterization of melanin pigments derived from Amorphotheca resinae |
Author |
Jeong-Joo Oh1, Jee Young Kim1, Sun Lul Kwon1, Dong-Hyeok Hwang2, Yoon-E Choi1, and Gyu-Hyeok Kim1* |
Address |
1Division of Environmental Science & Ecological Engineering, College of Life Sciences & Biotechnology, Korea University, Seoul 02841, Republic of Korea, 2Department of Biotechnology, College of Life Sciences & Biotechnology, Korea University, Seoul 02841, Republic of Korea |
Bibliography |
Journal of Microbiology, 58(8),648–656, 2020,
|
DOI |
10.1007/s12275-020-0054-z
|
Key Words |
Amorphotheca resinae, melanin pigments, fungal
melanins |
Abstract |
As melanin has emerged as functional pigment with cosmetic,
health and food applications, the demand for the pigments
is expected to increase. However, the conventional sources
(e.g. mushroom, hair, and wool) of melanin production entail
pigments inside the substrates which requires the costly
extraction procedures, leading to inappropriate scalable production.
In this study, we screened 102 of fungal isolates for
their ability to produce melanin in the supernatant and selected
the only Amorphotheca resinae as a promising candidate.
In the peptone yeast extract glucose broth, A. resinae
produced the melanin rapidly during the autolysis phase of
growth, reaching up 4.5 g/L within 14 days. Structural characterization
of the purified melanin from A. resinae was carried
out by using elemental analysis, electron paramagnetic
resonance, 13C solid-state nuclear magnetic resonance spectroscopy,
and pyrolysis-gas chromatography-mass spectrometry
in comparison with the standard melanins. The results
indicate that the structural properties of A. resinae melanin
is similar to the eumelanin which has a wide range of industrial
uses. For example, the purified melanin from A. resinae
has the potent antioxidant activities as a result of free
radical scavenging assays. Consequently, A. resinae KUC3009
can be a promising candidate for scalable production of industrially
applicable melanin. |