Title |
Changes in the microbial community of Litopenaeus vannamei larvae and rearing water during different growth stages after disinfection treatment of hatchery water |
Author |
Yafei Duan1,2, Yapeng Tang1,2, Jianhua Huang1,2, Jiasong Zhang1,2, Heizhao Lin1,2, Shigui Jiang2, Ruixuan Wang2, and Guofu Wang3 |
Address |
1Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518121, P. R. China, 2Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, P. R. China, 3Hainan Academy of Ocean and Fisheries Sciences, Haikou 571126, P. R. China |
Bibliography |
Journal of Microbiology, 58(9),741–749, 2020,
|
DOI |
10.1007/s12275-020-0053-0
|
Key Words |
chemical disinfectants, growth stages, Litopenaeus
vannamei larvae, microbial community, water |
Abstract |
Microbial communities greatly affect rearing water quality
and the larvae health during shrimp hatchery periods. In this
study, we investigated the microbial communities of rearing
water and larvae of Litopenaeus vannamei after treating hatchery
water with different kinds of chemical disinfectants: no
disinfectants (Con), chlorine dioxide (ClO2), formaldehyde
solution (HCHO), bleach powder (CaClO), and iodine (I2).
The water and larval samples were collected from nauplius 6
(N6), zoea 1 (Z1), mysis 1 (M1), and postlarvae 1 (P1) shrimp
growth periods. 16S rDNA high-throughput sequencing revealed
that the bacterial composition of the rearing water was
more complex than that of the larvae, and the bacterial community
of the rearing water and the larvae fluctuated significantly
at the P1 and Z1 periods, respectively. Disinfectants
altered the bacterial diversity and composition of the rearing
water and larvae. Specifically, in the rearing water of the
P1 period, Proteobacteria abundance was increased in the
HCHO group; while Bacteroidetes abundance was decreased
in the ClO2, HCHO, and I2 groups but increased in the CaClO
group. In the larvae of the Z1 period, Firmicutes (especially
Bacillus class) abundance was increased in the CaClO group,
but decreased in the ClO2, HCHO, and I2 groups. Network
analyses revealed that the genera Donghicola, Roseibacterium,
Candidatus-Cquiluna, and Nautella were enriched in the rearing
water, while Halomonas, Vibrio, and Flavirhabdus had
high abundance in the larvae. The survival of shrimp was influenced
by disinfectants that were inconsistent with the bacterial
community changes. These results will be helpful for
using microbial characteristics to facilitate healthy shrimp
nursery. |