Title |
Georgenia faecalis sp. nov. isolated from the faeces of Tibetan antelope |
Author |
Xiaoxia Wang1,2, Jing Yang2,3,4, Yuyuan Huang5, Xiaomin Wu6, Licheng Wang1, Limei Han1, Sha Li1, Huan Li1, Xiaoying Fu1, Hai Chen1, and Xiong Zhu1* |
Address |
1Central & Clinical Laboratory of Sanya People’s Hospital, Sanya, Hainan 572000, P. R. China, 2State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, P. R. China, 3Shanghai Institute for Emerging and Re-emerging Infectious Diseases, Shanghai Public Health Clinical Center, Shanghai 201508, P. R. China, 4Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing 100730, P. R. China, 5Guangxi Key Laboratory of AIDS Prevention and Treatment & Guangxi Collaborative Innovation Center for Biomedicine, School of Public Health, Guangxi Medical University, Nanning 530021, Guangxi, P. R. China, 6Shaanxi Institute of Zoology, Xian 710032, P. R. China |
Bibliography |
Journal of Microbiology, 58(9),734–740, 2020,
|
DOI |
10.1007/s12275-020-0060-1
|
Key Words |
Georgenia faecalis sp. nov., Tibetan antelope, Qinghai-
Tibet Plateau |
Abstract |
Two aerobic, Gram-stain-positive, non-motile, non-sporulating
coccoid strains, designated ZLJ0423T and ZLJ0321,
were isolated from the faeces of Tibetan antelope (Pantholops
hodgsonii). Their optimal temperature, NaCl concentration
and pH for growth were 28°C, 0.5% (w/v) NaCl and pH 7.5,
respectively. Phylogenetic analysis based on 16S rRNA gene
sequences revealed that strains ZLJ0423T and ZLJ0321 were
very similar to each other (99.8%) and had a sequence similarity
of 97.0% with Georgenia satyanarayanai NBRC 107612T
and Georgenia subflava CGMCC 1.12782T. Phylogenomic
analysis based on 688 core genes indicated that these strains
formed a clade with G. satyanarayanai NBRC 107612T and
Georgenia wutianyii Z294T. The predominant cellular fatty
acids were anteiso-C15:0, anteiso-C15:1 A and C16:0. The major
menaquinone was MK-8(H4). The cell-wall amino acids consisted
of alanine, lysine, glycine and aspartic acid, with lysine
as the diagnostic diamino acid. Diphosphatidylglycerol,
phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol
mannosides and two unidentified lipids formed the
polar lipid profile. The DNA G + C content of both isolates
was 73.9 mol%. The digital DNA–DNA hybridization value
between strains ZLJ0423T and ZLJ0321 was 91.2%, but their
values with closely related species and other available type
strains of the genus Georgenia were lower than the 70% threshold.
On the basis of polyphasic taxonomic data, strains
ZLJ0423T and ZLJ0321 represent a novel species within the
genus Georgenia, for which the name Georgenia faecalis sp.
nov. is proposed. The type strain is ZLJ0423T (= CGMCC
1.13681T = JCM 33470T). |