Title |
Fungal diversity in deep-sea sediments from Magellan seamounts environment of the western Pacific revealed by high-throughput Illumina sequencing |
Author |
Shuai Yang1, Wei Xu1, Yuanhao Gao1, Xiaoyao Chen4, and Zhu-Hua Luo1,2,3* |
Address |
1Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, 178 Daxue Road, Xiamen 361005, P. R. China, 2Co-Innovation Center of Jiangsu Marine Bioindustry Technology, Jiangsu Ocean University, Lianyungang 222005, P. R. China, 3School of Marine Sciences, Nanjing University of Information Science & Technology, Nanjing 210044, P. R. China, 4Monotoring Center of Fishery Resources, Fujian Province, Fuzhou 350003, P. R. China |
Bibliography |
Journal of Microbiology, 58(10),841–852, 2020,
|
DOI |
10.1007/s12275-020-0198-x
|
Key Words |
Magellan seamounts environment, high-throughput
Illumina sequencing, fungal diversity, western Pacific |
Abstract |
There are lots of seamounts globally whose primary production
is disproportionally greater than the surrounding areas.
Compared to other deep-sea environments, however, the
seamounts environment is relatively less explored for fungal
diversity. In the present study, we explored the fungal community
structure in deep-sea sediments from four different
stations of the Magellan seamounts environment by using
high-throughput sequencing of the ITS1 region. A total of
1,897,618 ITS1 sequences were obtained. Among these sequences,
fungal ITS1 sequences could be clustered into 1,662
OTUs. The majority of these sequences belonged to Ascomycota.
In the genera level, the most abundant genus was Mortierella
(4.79%), which was reported as a common fungal genus
in soil and marine sediments, followed by Umbelopsis
(3.80%), Cladosporium (2.98%), Saccharomycopsis (2.53%),
Aspergillus (2.42%), Hortaea (2.36%), Saitozyma (2.20%), Trichoderma
(2.12%), Penicillium (2.11%), Russula (1.86%), and
Verticillium (1.40%). Most of these recovered genera belong
to Ascomycota. The Bray-Curtis analysis showed that there
was 37 to 85% dissimilarity of fungal communities between
each two sediment samples. The Principal coordinates analysis
clearly showed variations in the fungal community among
different sediment samples. These results suggested that there
was a difference in fungal community structures not only
among four different sampling stations but also for different
layers at the same station. The depth and geographical distance
significantly affect the fungal community, and the effect of
depth and geographical distance on the structure of the fungal
community in the Magellan seamounts is basically same.
Most of the fungi were more or less related to plants, these
plant parasitic/symbiotic/endophytic fungi constitute a unique
type of seamounts environmental fungal ecology, different
from other marine ecosystems. |