Title |
Biosynthesis of adipic acid in metabolically engineered Saccharomyces cerevisiae |
Author |
Xi Zhang1,3, Yingli Liu2, Jing Wang2, Yunying Zhao1,4, and Yu Deng1,3,4* |
Address |
1National Engineering Laboratory for Cereal Fermentation Technology (NELCF), School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, P. R. China, 2China-Canada Joint Lab of Food Nutrition and Health (Beijing), Beijing Technology & Business University, Beijing 100048, P. R. China, 3School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, P. R. China, 4Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, P. R. China |
Bibliography |
Journal of Microbiology, 58(12),1065-1075, 2020,
|
DOI |
10.1007/s12275-020-0261-7
|
Key Words |
Saccharomyces cerevisiae, adipic acid, reverse adipate
degradation pathway (RADP), metabolic engineering,
fermentation |
Abstract |
Adipic Acid (AA) is a valued platform chemical compound,
which can be used as a precursor of nylon-6,6. Due to the
generation of an enormous amount of nitric oxide metabolites
and the growing depletion of oil resources as a result of
AA production from a mixture of cyclohexanol and cyclohexanone,
the microbial methods for synthesizing AA have
attracted significant attention. Of the several AA-producing
pathways, the reverse adipate degradation pathway in
Thermobifida fusca (Tfu RADP) is reported to be the most
efficient, which has been confirmed in Escherichia coli. In this
study, the heterologous Tfu RADP was constructed for producing
AA in S. cerevisiae by co-expressing genes of Tfu_
0875, Tfu_2399, Tfu_0067, Tfu_1647, Tfu_2576, and Tfu_
2576. The AA titer combined with biomass, cofactors and
other by-products was all determined after fermentation.
During batch fermentation in a shake flask, the maximum AA
titer was 3.83 mg/L, while the titer increased to 10.09 mg/L
during fed-batch fermentation in a 5-L bioreactor after fermentation
modification. |