Title |
Leucobacter coleopterorum sp. nov., Leucobacter insecticola sp. nov., and Leucobacter viscericola sp. nov., isolated from the intestine of the diving beetles, Cybister brevis and Cybister lewisianus, and emended description of the genus Leucobacter |
Author |
Dong-Wook Hyun, Hojun Sung, Pil Soo Kim, Ji-Hyun Yun, and Jin-Woo Bae* |
Address |
Department of Biology and Department of Biomedical and Pharmaceutical Sciences, Kyung Hee University, Seoul 02447, Republic of Korea |
Bibliography |
Journal of Microbiology, 59(4),360–368, 2021,
|
DOI |
10.1007/s12275-021-0472-6
|
Key Words |
Leucobacter, Leucobacter coleopterorum, Leucobacter
insecticola, Leucobacter viscericola, Cybister brevis,
Cybister lewisianus, diving beetle |
Abstract |
Three novel bacterial strains, HDW9AT, HDW9BT, and
HDW9CT, isolated from the intestine of the diving beetles
Cybister lewisianus and Cybister brevis, were characterized as
three novel species using a polyphasic approach. The isolates
were Gram-staining-positive, strictly aerobic, non-motile,
and rod-shaped. They grew optimally at 30°C (pH 7) in the
presence of 0.5% (wt/vol) NaCl. Phylogenetic analysis based
on the 16S rRNA gene sequences revealed that they belong
to the genus Leucobacter and are closely related to L. denitrificans
M1T8B10T (98.4–98.7% sequence similarity). Average
nucleotide identity (ANI) values among the isolates were
76.4–84.1%. ANI values for the isolates and the closest taxonomic
species, L. denitrificans KACC 14055T, were 72.3–73.1%.
The isolates showed ANI values of < 76.5% with all analyzable
Leucobacter strains in the EzBioCloud database. The
genomic DNA G + C content of the isolates was 60.3–62.5%.
The polar lipid components were phosphatidylglycerol, diphosphatidylglycerol,
and other unidentified glycolipids, phospholipids,
and lipids. The major cellular fatty acids were anteiso-
C15:0, iso-C16:0, and anteiso-C17:0. MK-10 was the major
respiratory quinone, and MK-7 and MK-11 were the minor
respiratory quinones. The whole-cell sugar components of the
isolates were ribose, glucose, galactose, and mannose. The
isolates harbored L-2,4-diaminobutyric acid, L-serine, L-lysine,
L-aspartic acid, glycine, and D-glutamic acid within the
cell wall peptidoglycan. Based on phylogenetic, phenotypic,
chemotaxonomic, and genotypic analyses, strains HDW9AT,
HDW9BT, and HDW9CT represent three novel species within
the genus Leucobacter. We propose the name Leucobacter
coleopterorum sp. nov. for strain HDW9AT (= KACC 21331T
= KCTC 49317T = JCM 33667T), the name Leucobacter insecticola
sp. nov. for strain HDW9BT (= KACC 21332T =
KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola
sp. nov. for strain HDW9CT (= KACC 21333T =
KCTC 49319T = JCM 33669T). |